NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Non-enzymatic Single-Cell Dispersion of normal or tumor tissue   Add to my SOP compendium

BRD ID: 1,085

Source: OSUCCC LTB Tier: SOP Number of times downloaded: 646

Description

This document describes the procedures used by the OSUCCC Leukemia Tissue Bank for tissue dissociation, cell isolation and harvest, and cell viability testing using the trypan blue dye exclusion method. It is a product of the OSUCCC Leukemia Tissue Bank (http://cancer.osu.edu/research-and-education/shared-resources/leukemia-tissue-bank).

Documents for Download

OSU LTB_ Single cell dispersion of small fresh tissue v1.0.pdf [Size: 251 KB]

Biospecimens
  • Tissue - General
Diagnoses
  • Normal
  • Neoplastic
Topics
  • Biospecimen Processing
  • Platform-specific Methodology

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