NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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Microbiome viability assay   Add to my SOP compendium

BRD ID: 3,070

Source: Immune Tolerance Network Tier: SOP Number of times downloaded: 60

Description

This document describes the procedures for fluorescence-activated cell sorting (FACS) in conjunction with viability fluorescent markers and metagenomic sequencing for the enumeration of total and viable bacterial cells and their taxonomic identification in maternal vaginal specimens. It is a product of the Immune Tolerance Network (ITN) and can be found at https://www.immunetolerance.org/researchers/lab-protocols.

Documents for Download

ITN079AD ACTIVATE_EP262 Microbiome viability assessment SOP.pdf [Size: 397 KB]
Biospecimens
  • Cell - Vagina
Diagnoses
  • Pregnant
Topics
  • Platform-specific Methodology

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