NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

On-site PBMC Isolation Using SepMate Tubes Standard Operating Procedure   Add to my SOP compendium

BRD ID: 3,065

Source: Immune Tolerance Network Tier: SOP Number of times downloaded: 142

Description

This document describes the procedures for the isolation and cryopreservation of peripheral blood mononuclear cells (PBMCs) from blood collected in heparin tubes. It is a product of the Immune Tolerance Network (ITN); the protocol can be found at https://www.immunetolerance.org/researchers/lab-protocols, and a video demonstrating the procedure can be viewed at https://youtu.be/ivs5ZxN-I_Q?si=I6OsXn8WyarPwcPN.

Documents for Download

ITN_ ITN091AI VIBRANT Onsite SepMate PBMC Isolation SOP.pdf [Size: 308 KB]

Biospecimens
  • Cell - White Cells
  • Fluid - Blood
Diagnoses
  • Not specified
Topics
  • Biospecimen Processing
  • Biospecimen Preservation
  • Storage

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