Semi-quantitative RT-PCR assessment of molecular markers in breast large-core needle biopsies.

Author(s): Pohlodek K, Galbavý S, Bartosová M, Mucha V, Holomán K, Pastorek J, Pastoreková S

Publication: Neoplasma, 2004, Vol. 51, Page 415-21

PubMed ID: 15640949 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effect of using LCNB rather than SE specimens for the determination of molecular marker status of breast tumors. Positive IHC staining was considered to be staining of more than 5% of neoplastic cells. Positive RT-PCR results were when levels of the marker were greater than 5% of the levels of beta 2 microglobulin (B2-M).

   Summary of Findings:

   The authors report that tumor stage and grade from LCNB specimens corresponded with those in SE specimens. For ER status, a concordance of 70.9% was found between IHC staining of FFPE SE and RT-PCR of frozen SE specimens. Concordance rates of ER, PR, HER-2, HER-3 and CD44 status between LCNB and SE frozen specimens by RT-PCR were 73.9%, 78%, 78%, 78% and 82%, respectively. The LCNB specimen was negative while the SE specimen was positive in only 1 case of less for all markers. The rest of the discordance came from positive LCNB specimens and negative SE specimens.

   Biospecimens

   • Tissue - Breast

   Preservative Types

   • Frozen
   • Formalin

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Protein	Immunohistochemistry
   RNA	RT-PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Formalin (buffered) Frozen
   RT-PCR Specific	Technology platform	IHC
   RT-PCR Specific	Targeted nucleic acid	ER B2-M PR HER-2 HER-3 CD44
   Immunohistochemistry Specific	Targeted peptide/protein	ER
   Biospecimen Acquisition	Method of tissue acquisition	Core needle biopsy Surgical resection

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