Pre-analytical Factor | Classification | Bodily fluids | Cells/Tissue | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Blood | Serum | Plasma | Urine | Saliva | |||||||
Clearing agent | Biospecimen Preservation | 0 | 0 | 0 | 0 | 0 | 9 | ||||
Clearing duration/condition | Biospecimen Preservation | 0 | 0 | 0 | 0 | 0 | 12 | ||||
Concentration of fixative | Biospecimen Preservation | 3 | 0 | 0 | 2 | 1 | 13 | ||||
Conditions during fixation/preservation | Biospecimen Preservation | 2 | 0 | 0 | 0 | 0 | 0 | ||||
Cooling or freezing method/ rate | Biospecimen Preservation | 8 | 1 | 8 | 3 | 2 | 29 | ||||
Dehydration duration/condition | Biospecimen Preservation | 1 | 1 | 2 | 0 | 0 | 14 | ||||
Dehydration reagent | Biospecimen Preservation | 0 | 0 | 0 | 0 | 0 | 6 | ||||
Duration of tissue/ specimen processing | Biospecimen Preservation | 1 | 0 | 1 | 0 | 0 | 20 | ||||
Embedding duration/condition | Biospecimen Preservation | 0 | 0 | 0 | 0 | 0 | 13 | ||||
Embedding medium | Biospecimen Preservation | 0 | 0 | 0 | 0 | 0 | 26 | ||||
Enzyme inhibitor | Biospecimen Preservation | 0 | 0 | 0 | 1 | 0 | 1 | ||||
Fixative additive/buffer | Biospecimen Preservation | 16 | 4 | 8 | 8 | 2 | 18 | ||||
Fixative pH | Biospecimen Preservation | 0 | 0 | 0 | 0 | 0 | 5 | ||||
Kinase inhibitor | Biospecimen Preservation | 0 | 0 | 0 | 0 | 0 | 1 | ||||
Method of fixative delivery | Biospecimen Preservation | 0 | 0 | 0 | 0 | 0 | 23 | ||||
Method of fixative removal | Biospecimen Preservation | 0 | 0 | 0 | 0 | 0 | 1 | ||||
RNA stabilization method | Biospecimen Preservation | 9 | 1 | 1 | 0 | 0 | 4 | ||||
Temperature of fixation/preservation | Biospecimen Preservation | 2 | 0 | 1 | 0 | 0 | 26 | ||||
Time in fixative | Biospecimen Preservation | 1 | 0 | 0 | 0 | 0 | 160 | ||||
Tissue to fixative ratio | Biospecimen Preservation | 0 | 0 | 0 | 0 | 0 | 2 | ||||
Type of fixation/preservation | Biospecimen Preservation | 239 | 93 | 156 | 90 | 12 | 535 | ||||
Volume/ concentration of preservative | Biospecimen Preservation | 0 | 0 | 0 | 0 | 0 | 1 |