Pre-analytical Factor | Classification | Bodily fluids | Cells/Tissue | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Blood | Serum | Plasma | Urine | Saliva | |||||||
Detection method | Platform Specific Methodology - RT-PCR | 0 | 0 | 0 | 0 | 0 | 0 | ||||
Incubation time | Platform Specific Methodology - RT-PCR | 0 | 0 | 0 | 0 | 0 | 1 | ||||
Length of gene fragment | Platform Specific Methodology - RT-PCR | 0 | 0 | 0 | 1 | 0 | 35 | ||||
Number of cycles | Platform Specific Methodology - RT-PCR | 0 | 0 | 0 | 0 | 0 | 1 | ||||
Priming method | Platform Specific Methodology - RT-PCR | 0 | 0 | 0 | 0 | 0 | 2 | ||||
Reaction solution | Platform Specific Methodology - RT-PCR | 1 | 0 | 0 | 0 | 0 | 2 | ||||
RNA incubation temperature | Platform Specific Methodology - RT-PCR | 0 | 0 | 0 | 0 | 0 | 4 | ||||
Targeted nucleic acid | Platform Specific Methodology - RT-PCR | 19 | 3 | 7 | 1 | 0 | 50 | ||||
Technology platform | Platform Specific Methodology - RT-PCR | 4 | 1 | 4 | 0 | 0 | 3 | ||||
Template modification | Platform Specific Methodology - RT-PCR | 1 | 0 | 1 | 0 | 0 | 1 | ||||
Type of tissue stain | Platform Specific Methodology - RT-PCR | 0 | 0 | 0 | 1 | 0 | 1 |