The effect of decalcification on in situ hybridization.

Author(s): Arber JM, Weiss LM, Chang KL, Battifora H, Arber DA

Publication: Mod Pathol, 1997, Vol. 10, Page 1009-14

PubMed ID: 9346180 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of decalcifying agent and fixation duration on ISH staining. Non malignant adrenal gland, brain, breast, colon, heart, kidney, liver, lung, lymph, nerve, pancreas, prostate, skin, skeletal muscle, spleen, stomach, thyroid, and uterus as well as lymphoma, melanoma, carcinoid tumor, undifferentiated sarcoma, and angiosarcoma specimens were compared.

   Summary of Findings:

   Staining of versenate-decalcified specimens was positive in 93% of specimens with 72% staining equal to or more intensely than non-decalcified controls. Staining of S/P-decalcified specimens was more variable, and only 49% of specimens had staining more intense or equal to non-decalcified controls. RBD-decalcified specimens had very low staining with only 8% staining equally to or more intensely than non-decalcified controls and 25% not staining at all. Formalin-fixation duration and tissue type did not have any clear effects.

   Biospecimens

   • Tissue - Adrenal Gland
   • Tissue - Breast
   • Tissue - Brain
   • Tissue - Colorectal
   • Tissue - Heart
   • Tissue - Kidney
   • Tissue - Liver
   • Tissue - Lung
   • Tissue - Lymph Node
   • Tissue - Nerve
   • Tissue - Pancreas
   • Tissue - Prostate
   • Tissue - Skin
   • Tissue - Muscle (Skeletal)
   • Tissue - Spleen
   • Tissue - Stomach
   • Tissue - Thyroid Gland
   • Tissue - Uterus
   • Tissue - Other

   Preservative Types

   • Formalin

   Diagnoses:

   • Neoplastic - Lymphoma
   • Neoplastic - Sarcoma
   • Neoplastic - Carcinoma
   • Neoplastic - Melanoma
   • Neoplastic - Benign
   • Autopsy

   Platform:

   Analyte	Technology Platform
   RNA	In situ hybridization

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Analyte Extraction and Purification	Decalcification solution/ duration	Versenate (EDTA-based) S/P Decal (HCl based) RBD (HCl based)
   Biospecimen Preservation	Time in fixative	6 h 24 h 72 h
   Biospecimen Acquisition	Biospecimen location	Adrenal gland Breast Brain Colon Heart Kidney Liver Lung Lymph Nerve Pancreas Prostate Skin Skeletal muscle Spleen Stomach Thyroid Uterus

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