Pre-analytical Factor | Classification | Bodily fluids | Cells/Tissue | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Blood | Serum | Plasma | Urine | Saliva | |||||||
Analyte isolation method | Analyte Extraction and Purification | 106 | 40 | 118 | 33 | 5 | 193 | ||||
Analyte purification | Analyte Extraction and Purification | 14 | 7 | 11 | 5 | 2 | 19 | ||||
Antigen retrieval | Analyte Extraction and Purification | 0 | 0 | 0 | 1 | 0 | 56 | ||||
Between Site Variability | Analyte Extraction and Purification | 1 | 0 | 0 | 0 | 0 | 0 | ||||
Cell/tissue permeabilization | Analyte Extraction and Purification | 4 | 0 | 0 | 0 | 0 | 12 | ||||
Decalcification solution/ duration | Analyte Extraction and Purification | 0 | 0 | 0 | 1 | 0 | 23 | ||||
Deparaffinization | Analyte Extraction and Purification | 0 | 0 | 0 | 0 | 0 | 42 | ||||
Fat clearing | Analyte Extraction and Purification | 0 | 0 | 0 | 0 | 0 | 1 | ||||
Filtration of purified DNA | Analyte Extraction and Purification | 1 | 0 | 0 | 0 | 0 | 1 | ||||
HPLC elution time | Analyte Extraction and Purification | 0 | 0 | 0 | 0 | 0 | 0 | ||||
Hydrolyzation | Analyte Extraction and Purification | 0 | 0 | 0 | 0 | 0 | 0 | ||||
Incubation duration/condition | Analyte Extraction and Purification | 9 | 2 | 7 | 0 | 0 | 25 | ||||
Nucleic acid digestion | Analyte Extraction and Purification | 17 | 6 | 5 | 2 | 5 | 12 | ||||
Protease inhibitor | Analyte Extraction and Purification | 19 | 16 | 23 | 12 | 6 | 0 | ||||
Protein digestion | Analyte Extraction and Purification | 5 | 3 | 9 | 1 | 1 | 57 | ||||
Protein solubilization | Analyte Extraction and Purification | 1 | 1 | 0 | 0 | 0 | 5 | ||||
Rehydration of dried sample/specimen | Analyte Extraction and Purification | 5 | 1 | 7 | 0 | 2 | 7 | ||||
RNase inactivation | Analyte Extraction and Purification | 4 | 4 | 2 | 0 | 0 | 1 | ||||
Sample mixing | Analyte Extraction and Purification | 4 | 4 | 2 | 0 | 0 | 0 | ||||
Specimen format | Analyte Extraction and Purification | 1 | 0 | 1 | 0 | 0 | 2 | ||||
Temperature of heat-induced retrieval | Analyte Extraction and Purification | 1 | 0 | 0 | 0 | 0 | 23 | ||||
Tissue homogenization | Analyte Extraction and Purification | 0 | 0 | 0 | 0 | 0 | 3 | ||||
Washing | Analyte Extraction and Purification | 5 | 0 | 1 | 1 | 0 | 14 |