A novel fixative improves opportunities of nucleic acids and proteomic analysis in human archive's tissues.
Author(s): Stanta G, Mucelli SP, Petrera F, Bonin S, Bussolati G
Publication: Diagn Mol Pathol, 2006, Vol. 15, Page 115-23
PubMed ID: 16778593 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to compare protein antigenicity via immunohistochemistry among tissues preserved with FineFIX or formalin and paraffin embedded.
Summary of Findings:
Compared to formalin-fixed, paraffin-embedded tissue, tissue fixed in FineFIX with microwave assistance and embedded in paraffin yielded greater staining intensity while preserving appropriate staining patterns.
Biospecimens
Preservative Types
- Other Preservative
- Formalin
Diagnoses:
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform Protein Immunohistochemistry Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Preservation Type of fixation/preservation FineFIX
Formalin (buffered)
Biospecimen Preservation Method of fixative delivery Immersion
Microwaved
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Study Purpose
The purpose of this study was to compare RNA integrity in FineFIXed and formalin-fixed specimens that were paraffin embedded.
Summary of Findings:
Longer beta-actin amplicons were generated from RNA extracted from FineFixed, paraffin-embedded specimens (450 bp in 100% and 600 bp in 60% of cases) compared to RNA extracted from formalin-fixed, paraffin embedded specimens (170 bp).
Biospecimens
Preservative Types
- Other Preservative
- Formalin
Diagnoses:
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform RNA RT-PCR Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Preservation Type of fixation/preservation FineFIX
Formalin (buffered)
RT-PCR Specific Length of gene fragment 77 bp
170 bp
200 bp
438 bp
651 bp
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Study Purpose
The purpose of this study was to compare the preservation of DNA in FineFIXed and formalin-fixed specimens that were paraffin embedded.
Summary of Findings:
The authors reported greater total DNA yield from FineFIXed specimens compared to those fixed in formalin. The maximum TTR fragment length amplified from DNA isolated from formalin-fixed, paraffin-embedded tissue sections was 339bp, while tissue fixed in FineFIX and embedded paraffin yielded fragments as long as 2395 bp, the longest amplicon attempted.
Biospecimens
Preservative Types
- Other Preservative
- Formalin
Diagnoses:
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform DNA PCR Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Preservation Type of fixation/preservation FineFIX
Formalin (buffered)
PCR Specific Length of gene fragment 291 bp
339 bp
1000 bp
1939 bp
2395 bp
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Study Purpose
The purpose of this study was to determine if protein extraction and subsequent analysis was possible in FineFIXed specimens, and whether results were comparable to those observed in fresh frozen specimens.
Summary of Findings:
Of the approximately 1000 protein spots identified by 2D-gel analysis, only 1.4% differed among FineFIXed and fresh frozen specimens. Western blot analysis for alpha tubulin and human tTG yielded comparable results among FineFIXed and fresh frozen specimens, although a second smaller alpha tubulin protein band was observed in fresh frozen specimens, which the authors hypothesized to be a product of degradation. No proteins were observed in extracts from formalin-fixed specimens.
Biospecimens
Preservative Types
- Other Preservative
- Frozen
- Formalin
Diagnoses:
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform Protein 1D/2D gels Protein Western blot Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Preservation Type of fixation/preservation FineFIX
Formalin (buffered)
Snap frozen