Effects of anticoagulants on human plasma soluble corin levels measured by ELISA.

Author(s): Dong N, Dong J, Liu P, Xu L, Shi S, Wu Q

Publication: Clin Chim Acta, 2010, Vol. 411, Page 1998-2003

PubMed ID: 20800586 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of anticoagulant, gender, and age on the measurement of native corin in plasma and serum specimens.

   Summary of Findings:

   Use of sodium citrate or K2EDTA as anticoagulants resulted in significantly lower plasma corin levels when compared to levels measured in either serum or heparin-plasma, which were similar to each other. Corin levels were higher in males than in females when measured in K2EDTA-plasma (p<0.001). There were no significant differences found in K2EDTA-plasma corin levels based on age.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	ELISA

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum
   Preaquisition	Patient gender	Female Male
   Preaquisition	Patient age	16-30 y 31-45 y 46-60 y >60 y
   Biospecimen Acquisition	Anticoagulant	Heparin Potassium EDTA Sodium citrate None

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2. Study Purpose

   The purpose of this study was to determine the effects of storage temperature and duration, freeze-thaw cycling, and the addition of protease inhibitors on the stability of native and recombinant corin in plasma and serum specimens.

   Summary of Findings:

   After 24 hours at room temperature, corin levels in K2EDTA-plasma significantly decreased. However, at 4 degrees C, corin levels in K2EDTA-plasma remained stable for 72 hours. Recombinant human corin added to K2EDTA-plasma specimens exhibited stability similar to native corin at 4 degrees C, however, recombinant corin was stable at room temperature up to 48 hours. The addition of protease inhibitor cocktail did not affect native or recombinant corin stability at either temperature. Up to 3 freeze-thaw cycles had no effects on the stability of native corin in K2EDTA-plasma, sodium citrate-plasma, or serum. However, after 2 freeze-thaw cycles, corin levels significantly decreased in heparin-plasma (p<0.05).

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)
   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	ELISA

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	Room temperature 4 degrees C
   Storage	Storage duration	0 h 4 h 8 h 12 h 24 h 48 h 72 h
   Storage	Freeze/thaw cycling	0 cycles 1 cycle 2 cycles 3 cycles
   Analyte Extraction and Purification	Protease inhibitor	Cocktail No protease inhibitor added
   ELISA Specific	Targeted peptide/protein	Native corin Recombinant corin
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum
   Biospecimen Preservation	Type of fixation/preservation	Frozen None (fresh)
   Biospecimen Acquisition	Anticoagulant	Sodium citrate Potassium EDTA Heparin

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