A simple and rapid method for the detection of RNA in formalin-fixed, paraffin-embedded tissues by PCR amplification.
Author(s): von Weizsäcker F, Labeit S, Koch HK, Oehlert W, Gerok W, Blum HE
Publication: Biochem Biophys Res Commun, 1991, Vol. 174, Page 176-80
PubMed ID: 1703409 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of proteinase K digestion duration on the yield and amplificability of DNA and RNA from FFPE fine needle liver biopsies. After deparaffinization with xylene, sections were placed in lysis buffer containing proteinase K for 6-24 h, and nucleic acids were subsequently extracted using phenol chloroform.
Summary of Findings:
DNA was only apparent on the agarose gel after 24 h of digestion, but RNA was apparent for specimens digested for only 6 h. DNA and RNA were amplifiable from FFPE specimens, but the optimum digestion times were unspecified.
Biospecimens
Preservative Types
- Formalin
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform RNA Electrophoresis RNA RT-PCR DNA Electrophoresis DNA PCR Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) PCR Specific Targeted nucleic acid Albumin
RT-PCR Specific Targeted nucleic acid Albumin
Analyte Extraction and Purification Protein digestion 6 h
12 h
24 h