Use of the dried blood spot sampling process coupled with fast gas chromatography and negative-ion chemical ionization tandem mass spectrometry: application to fluoxetine, norfluoxetine, reboxetine, and paroxetine analysis.

Author(s): Déglon J, Lauer E, Thomas A, Mangin P, Staub C

Publication: Anal Bioanal Chem, 2010, Vol. 396, Page 2523-32

PubMed ID: 20063149 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage and extraction method on the recovery of antidepressants from spiked dried DBS by gas chromatography negative ion chemical ionization tandem MS (GC-NICI-MS-MS).

   Summary of Findings:

   Extraction of antidepressants with butyl chloride resulted in the best sensitivity and selectivity for NICI-MS-MS. At all concentrations, more than 78% of fluoxetine, norfluxetine, reboxetine, and paroxetine were recovered from the spiked DBS. Regardless of storage temperature, 85-115% of fluoxetine, norfluxetine, reboxetine, and paroxetine levels were recovered from spiked DBS after up to 30 days of storage. Using DBS, fluoxetine and norfluxetine could be detected in blood obtained from a single healthy individual 250 and 650 h, respectively, after administration of a single 40 mg dose of fluoxetine.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • Other Preservative
   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Small molecule	GC-MS

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	-20 degrees C 4 degrees C 25 degrees C 40 degrees C
   Storage	Storage duration	1 day 3 days 5 days 15 days 30 days
   Biospecimen Acquisition	Time of biospecimen collection	0 h after fluoxetine administration 6 h after fluoxetine administration 12 h after fluoxetine administration 24 h after fluoxetine administration 48 h after fluoxetine administration 72 h after fluoxetine administration 96 h after fluoxetine administration 120 h after fluoxetine administration 192 h after fluoxetine administration 250 h after fluoxetine administration 312 h after fluoxetine administration 480 h after fluoxetine administration 650 h after fluoxetine administration
   Analyte Extraction and Purification	Analyte isolation method	Ethyl acetate Hexane Butyl chloride
   Biospecimen Preservation	Type of fixation/preservation	Air-dried None (fresh)

   View more