NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Multiplex IHC Image Processing   Add to my SOP compendium

BRD ID: 2,897

Source: HTAN Tier: SOP Number of times downloaded: 29


This document describes an overview of the image processing pipeline for chromogenic multiplex immunohistochemistry (IHC) analysis of single tissue sections at Oregon Health and Science University as part of the National Cancer Institute's Human Tissue Atlas Network (HTAN; initiative. Instructions on file preparation, nuclei segmentation, and capture of single cell measurements are also provided. The SOP is available online via the HTAN workspace at ( and directly at

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NCI HTAN multiplex-ihc-image-processing-v0-2-cy9kxz4w.pdf [Size: 3400 KB]

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