Multiplex IHC Image Processing
BRD ID: 2,897
Source: HTAN Tier: SOP Number of times downloaded: 35
Description
This document describes an overview of the image processing pipeline for chromogenic multiplex immunohistochemistry (IHC) analysis of single tissue sections at Oregon Health and Science University as part of the National Cancer Institute's Human Tissue Atlas Network (HTAN; https://humantumoratlas.org/) initiative. Instructions on file preparation, nuclei segmentation, and capture of single cell measurements are also provided. The SOP is available online via the HTAN workspace at protocols.io (https://www.protocols.io/workspaces/ncihtan/publications) and directly at https://dx.doi.org/10.17504/protocols.io.n92ldmmznl5b/v2.
Documents for Download
NCI HTAN multiplex-ihc-image-processing-v0-2-cy9kxz4w.pdf [Size: 3400 KB]Biospecimens
- Tissue - General
Diagnoses
- Not specified
Topics
- Platform-specific Methodology
Associated SOP Compendiums
- Platform-specific Methodology