NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Targeted ExSeq -- Sequencing Library Preparation   Add to my SOP compendium

BRD ID: 2,895

Source: HTAN Tier: SOP Number of times downloaded: 35

Description

This document describes the procedures used for library preparation for targeted expansion sequencing (ExSeq) of samples that were processed using the Targeted ExSeq Tissue Preparation SOP. The SOP was used to profile metastatic breast cancer biopsies by the following participating centers of the National Cancer Institute's Human Tissue Atlas Network (HTAN; https://humantumoratlas.org/) as part of the pilot project: Massachusetts Institute of Technology, Harvard University, Broad Institute, and Howard Hughes Medical Institute. The SOP is available online via the HTAN workspace at protocols.io (https://www.protocols.io/workspaces/ncihtan/publications) and directly at https://dx.doi.org/10.17504/protocols.io.bgp7jvrn.

Documents for Download

NCI HTAN targeted-exseq-sequencing-library-preparation-bgp7jvrn.pdf [Size: 710 KB]

Biospecimens
  • Tissue - Breast
Diagnoses
  • Neoplastic - Carcinoma
Topics
  • Analyte Extraction and Purification
  • Platform-specific Methodology

Associated SOP Compendiums

News and Announcements

  • Most Popular SOPs in March 2024

  • New SOPs Available

  • Most Downloaded SOPs in January and February of 2024

  • More...