NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Targeted ExSeq -- Sequencing Library Preparation   Add to my SOP compendium

BRD ID: 2,895

Source: HTAN Tier: SOP Number of times downloaded: 35


This document describes the procedures used for library preparation for targeted expansion sequencing (ExSeq) of samples that were processed using the Targeted ExSeq Tissue Preparation SOP. The SOP was used to profile metastatic breast cancer biopsies by the following participating centers of the National Cancer Institute's Human Tissue Atlas Network (HTAN; as part of the pilot project: Massachusetts Institute of Technology, Harvard University, Broad Institute, and Howard Hughes Medical Institute. The SOP is available online via the HTAN workspace at ( and directly at

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NCI HTAN targeted-exseq-sequencing-library-preparation-bgp7jvrn.pdf [Size: 710 KB]

  • Tissue - Breast
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