NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Targeted ExSeq -- Tissue Preparation V.2   Add to my SOP compendium

BRD ID: 2,891

Source: HTAN Tier: SOP Number of times downloaded: 35

Description

This document describes tissue fixation, processing, sectioning, digestion, expansion, passivation, and specimen trimming for targeted expansion sequencing (ExSeq) of paraformaldehyde-fixed and snap-frozen tissue sections used by the following participating centers of the National Cancer Institute's Human Tissue Atlas Network (HTAN; https://humantumoratlas.org/) initiative: Massachusetts Institute of Technology, Harvard University, Bar-Ilan University, and Howard Hughes Medical Institute. The SOP is available online via the HTAN workspace at protocols.io (https://www.protocols.io/workspaces/ncihtan/publications) and directly at https://dx.doi.org/10.17504/protocols.io.14egn7w6zv5d/v1.

Documents for Download

NCI HTAN targeted-exseq-tissue-preparation-b2emqbc6.pdf [Size: 1101 KB]

Biospecimens
  • Tissue - General
Diagnoses
  • Not specified
Topics
  • Biospecimen Preservation
  • Biospecimen Processing
  • Analyte Extraction and Purification

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