Targeted ExSeq -- Tissue Preparation V.2
BRD ID: 2,891
Source: HTAN Tier: SOP Number of times downloaded: 41
Description
This document describes tissue fixation, processing, sectioning, digestion, expansion, passivation, and specimen trimming for targeted expansion sequencing (ExSeq) of paraformaldehyde-fixed and snap-frozen tissue sections used by the following participating centers of the National Cancer Institute's Human Tissue Atlas Network (HTAN; https://humantumoratlas.org/) initiative: Massachusetts Institute of Technology, Harvard University, Bar-Ilan University, and Howard Hughes Medical Institute. The SOP is available online via the HTAN workspace at protocols.io (https://www.protocols.io/workspaces/ncihtan/publications) and directly at https://dx.doi.org/10.17504/protocols.io.14egn7w6zv5d/v1.
Documents for Download
NCI HTAN targeted-exseq-tissue-preparation-b2emqbc6.pdf [Size: 1101 KB]Biospecimens
- Tissue - General
Diagnoses
- Not specified
Topics
- Biospecimen Preservation
- Biospecimen Processing
- Analyte Extraction and Purification
Associated SOP Compendiums
- Biospecimen Preservation
- Biospecimen Processing
- Analyte Extraction and Purification