NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Targeted ExSeq -- Tissue Preparation V.2   Add to my SOP compendium

BRD ID: 2,891

Source: HTAN Tier: SOP Number of times downloaded: 35


This document describes tissue fixation, processing, sectioning, digestion, expansion, passivation, and specimen trimming for targeted expansion sequencing (ExSeq) of paraformaldehyde-fixed and snap-frozen tissue sections used by the following participating centers of the National Cancer Institute's Human Tissue Atlas Network (HTAN; initiative: Massachusetts Institute of Technology, Harvard University, Bar-Ilan University, and Howard Hughes Medical Institute. The SOP is available online via the HTAN workspace at ( and directly at

Documents for Download

NCI HTAN targeted-exseq-tissue-preparation-b2emqbc6.pdf [Size: 1101 KB]

  • Tissue - General
  • Not specified
  • Biospecimen Preservation
  • Biospecimen Processing
  • Analyte Extraction and Purification

Associated SOP Compendiums

News and Announcements

  • Most Popular SOPs in March 2024

  • New SOPs Available

  • Most Downloaded SOPs in January and February of 2024

  • More...