NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

WU sn-prep Protocol for Solid Tumors - snRNA protocol v2.8   Add to my SOP compendium

BRD ID: 2,890

Source: HTAN Tier: SOP Number of times downloaded: 88

Description

This document describes the procedure for single nucleus preparation from frozen tissue or pulverized powder for single nucleus sequencing (sn-Seq) that is used by Washington University, a participating center of the National Cancer Institute's Human Tissue Atlas Network (HTAN; https://humantumoratlas.org/) initiative. The SOP is available online via the HTAN workspace at protocols.io (https://www.protocols.io/workspaces/ncihtan/publications) and directly at https://dx.doi.org/10.17504/protocols.io.14egn7w6zv5d/v1.

Documents for Download

NCI HTAN wu-sn-prep-protocol-for-solid-tumors-snrna-protoco-b72jrqcn.pdf [Size: 75 KB]

Biospecimens
  • Tissue - General
Diagnoses
  • Not specified
Topics
  • Biospecimen Processing

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