NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Tissue Cyclic Immunofluorescence (t-CyCIF)   Add to my SOP compendium

BRD ID: 2,889

Source: HTAN Tier: SOP Number of times downloaded: 58

Description

This document describes the multiplex procedure for tissue-based cyclic immunofluorescence (t-CyCIF) staining and imaging of glass-mounted paraffin-embedded sections used by Harvard Medical School and Brigham and Women's Hospital as part of the National Cancer Institute's Human Tissue Atlas Network (HTAN; https://humantumoratlas.org/) initiative. The SOP is available online via the HTAN workspace at protocols.io (https://www.protocols.io/workspaces/ncihtan/publications) and directly at https://dx.doi.org/10.17504/protocols.io.5qpvorbndv4o/v2.

Documents for Download

NCI HTAN tissue-cyclic-immunofluorescence-t-cycif-version-3-ch2ht8b6.pdf [Size: 432 KB]

Biospecimens
  • Tissue - General
Diagnoses
  • Not specified
Topics
  • Platform-specific Methodology

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