Tissue Cyclic Immunofluorescence (t-CyCIF)
BRD ID: 2,889
Source: HTAN Tier: SOP Number of times downloaded: 198
Description
This document describes the multiplex procedure for tissue-based cyclic immunofluorescence (t-CyCIF) staining and imaging of glass-mounted paraffin-embedded sections used by Harvard Medical School and Brigham and Women's Hospital as part of the National Cancer Institute's Human Tissue Atlas Network (HTAN; https://humantumoratlas.org/) initiative. The SOP is available online via the HTAN workspace at protocols.io (https://www.protocols.io/workspaces/ncihtan/publications) and directly at https://dx.doi.org/10.17504/protocols.io.5qpvorbndv4o/v2.
Documents for Download
NCI HTAN tissue-cyclic-immunofluorescence-t-cycif-version-3-ch2ht8b6.pdf [Size: 432 KB]Biospecimens
- Tissue - General
Diagnoses
- Not specified
Topics
- Platform-specific Methodology
Associated SOP Compendiums
- Platform-specific Methodology