NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Tissue Cyclic Immunofluorescence (t-CyCIF)   Add to my SOP compendium

BRD ID: 2,889

Source: HTAN Tier: SOP Number of times downloaded: 58


This document describes the multiplex procedure for tissue-based cyclic immunofluorescence (t-CyCIF) staining and imaging of glass-mounted paraffin-embedded sections used by Harvard Medical School and Brigham and Women's Hospital as part of the National Cancer Institute's Human Tissue Atlas Network (HTAN; initiative. The SOP is available online via the HTAN workspace at ( and directly at

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NCI HTAN tissue-cyclic-immunofluorescence-t-cycif-version-3-ch2ht8b6.pdf [Size: 432 KB]

  • Tissue - General
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  • Platform-specific Methodology

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