Cell preparation for scRNA-Seq from diluted bodily fluids V.2
BRD ID: 2,887
Source: HTAN Tier: SOP Number of times downloaded: 34
Description
The document describes the procedures used by the Memorial Sloan Kettering Cancer Center and the National Cancer Institute's Human Tissue Atlas Network initiative (HTAN; https://humantumoratlas.org/) to isolate, concentrate, and count viable primary immune and cancer cells from a diluted suspension of bodily fluid, such as cerebrospinal fluid. The SOP is available online via the HTAN workspace at protocols.io (https://www.protocols.io/workspaces/ncihtan/publications) and directly at https://dx.doi.org/10.17504/protocols.io.ewov1o7wolr2/v1.
Documents for Download
NCI HTAN V2 cell-preparation-for-scrna-seq-from-diluted-bodily-cirxud7n.pdf [Size: 155 KB]Biospecimens
- Fluid - Cerebrospinal Fluid
- Fluid - General
Diagnoses
- Neoplastic - Carcinoma
- Not specified
Topics
- Biospecimen Processing
- Platform-specific Methodology
Associated SOP Compendiums
- Biospecimen Processing
- Platform-specific Methodology