NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Cell preparation for scRNA-Seq from diluted bodily fluids V.2   Add to my SOP compendium

BRD ID: 2,887

Source: HTAN Tier: SOP Number of times downloaded: 31

Description

The document describes the procedures used by the Memorial Sloan Kettering Cancer Center and the National Cancer Institute's Human Tissue Atlas Network initiative (HTAN; https://humantumoratlas.org/) to isolate, concentrate, and count viable primary immune and cancer cells from a diluted suspension of bodily fluid, such as cerebrospinal fluid. The SOP is available online via the HTAN workspace at protocols.io (https://www.protocols.io/workspaces/ncihtan/publications) and directly at https://dx.doi.org/10.17504/protocols.io.ewov1o7wolr2/v1.

Documents for Download

NCI HTAN V2 cell-preparation-for-scrna-seq-from-diluted-bodily-cirxud7n.pdf [Size: 155 KB]

Biospecimens
  • Fluid - Cerebrospinal Fluid
  • Fluid - General
Diagnoses
  • Neoplastic - Carcinoma
  • Not specified
Topics
  • Biospecimen Processing
  • Platform-specific Methodology

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