NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

WUSTL Senescence Network (SenNet) Biospecimen Collection SOP   Add to my SOP compendium

BRD ID: 2,885

Source: HTAN Tier: SOP Number of times downloaded: 30

Description

This document describes procedures that are used at Washington University's Biospecimen Core as part of the Cellular Senescence Network (SenNEt) and the National Cancer Institute's Human Tissue Atlas Network (HTAN; https://humantumoratlas.org/) initiative for the collection, division, and preservation of skin, liver, and bone marrow tissue specimens by a slow freeze method (OCT embedding and freezing on dry ice), snap-freezing in liquid nitrogen, and formalin fixation. It also addresses the storage of preserved tissue specimens until analysis. The document also includes procedures for the collection and processing of EDTA plasma and serum specimens, isolation of peripheral blood mononuclear cells (PBMCs), and frozen storage of these fluid and isolated cell specimens. The SOP is available online via the HTAN workspace at protocols.io (https://www.protocols.io/workspaces/ncihtan/publications) and directly at https://dx.doi.org/10.17504/protocols.io.j8nlkww45l5r/v1.

Documents for Download

NCI HTAN wustl-senescence-network-sennet-biospecimen-collec-cimhuc36.pdf [Size: 169 KB]

Biospecimens
  • Tissue - Skin
  • Tissue - Liver
  • Tissue - Bone Marrow
  • Fluid - Blood
  • Fluid - Plasma
  • Fluid - Serum
  • Cell - White Cells
Diagnoses
  • Not specified
Topics
  • Biospecimen Acquisition
  • Biospecimen Processing
  • Biospecimen Preservation
  • Storage

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