TST Nuclei Isolation with GentleMACS
BRD ID: 2,884
Source: HTAN Tier: SOP Number of times downloaded: 45
Description
This document describes procedures used by the Dana Farber Cancer Institute to isolate nuclei from frozen tissue specimens as part of the National Cancer Institute's Human Tissue Atlas Network (HTAN, https://humantumoratlas.org/) initiative. It has been used in conjunction with the HTAN single-nuclei RNA-seq preparation protocol for analysis of frozen melanoma, breast, and lung metastases. The SOP is available online via the HTAN workspace at protocols.io (https://www.protocols.io/workspaces/ncihtan/publications) and directly at https://dx.doi.org/10.17504/protocols.io.dm6gp39z1vzp/v1.
Documents for Download
NCI HTAN tst-nuclei-isolation-with-gentlemacs-220301-czwzx7f6.pdf [Size: 145 KB]Biospecimens
- Tissue - Skin
- Tissue - Breast
- Tissue - Lung
Diagnoses
- Neoplastic - Melanoma
- Neoplastic - Carcinoma
Topics
- Biospecimen Processing
Associated SOP Compendiums
- Biospecimen Processing