Determination of intracellular reactive oxygen species (EUNCL-GTA-010)
BRD ID: 2,431
Source: EUNCL Tier: SOP Number of times downloaded: 1568
Description
This document describes procedures for culturing human hepatocarcinoma cells (Hep G2) and adenocarcinomic human alveolar basal epithelial cells (A549) and quantification of reactive oxygen species generated in response to nanoparticles (NPs) using the High Content Screening (HCS) platform by automated imaging and subsequent multiparametric image analysis. This SOP is a product of the European Nanomedicine Characterisation Laboratory (EUNCL) and is available from https://www.euncl.org/. THE URL SPECIFIED IS A LEGACY WEBSITE. NCI IS NO LONGER IN CONTACT WITH THE SOURCE THAT CONTRIBUTED THIS SOP.
Documents for Download
EUNCL_GTA_010.pdf [Size: 2288 KB]Biospecimens
- Cell - Liver
- Cell - Lung
Diagnoses
- General
Topics
- Biospecimen Processing
- Platform-specific Methodology
Associated SOP Compendiums
- Biospecimen Processing
- Platform-specific Methodology