Preparation of blood and PBMC for cytokine secretion (EUNCL-ITA-010)
BRD ID: 2,427
Source: EUNCL Tier: SOP Number of times downloaded: 1980
Description
This document describes the procedures for the isolation of white cells from whole blood collected in lithium heparin tubes by Ficoll-Paque density gradient centrifugation, and quantification of white cell cytokine production via ELISA in response to nanoparticle exposure. This SOP is a product of the European Nanomedicine Characterisation Laboratory (EUNCL) and is available at: http://www.euncl.org/about-us/assay-cascade/. THE URL SPECIFIED IS A LEGACY WEBSITE. NCI IS NO LONGER IN CONTACT WITH THE SOURCE THAT CONTRIBUTED THIS SOP.
Documents for Download
EUNCL_ITA_010.pdf [Size: 447 KB]Biospecimens
- Fluid - Blood
- Cell - White Cells
Diagnoses
- General
Topics
- Biospecimen Processing
- Platform-specific Methodology
Associated SOP Compendiums
- Biospecimen Processing
- Platform-specific Methodology