Office of Cancer Genomics (OCG) Burkitt Lymphoma Genome Sequencing Project (BLGSP) Standard Operating Procedures (SOP) Manual  

This document describes the procedures used by the Burkitt Lymphoma Genome Sequencing Project (BLGSP; https://www.cancer.gov/ccg/research/genome-sequencing/cgci/about/projects#burkitt-lymphoma-genome-sequencing-project-blgsp) for the collection, processing, preservation, and shipment of blood, buccal cell, and tissue specimens. Specific procedures included in the document include instruction for cell and tissue preservation by snap-freezing and/or formalin fixation; processing FFPE tissue scrolls; generating a peripheral blood smear; isolation of white blood cells and granulocytes with and without blood fractionation; creation of tissue microarrays; hematoxylin and eosin (H&E) staining, immunohistochemical staining, and fluorescence in situ hybridization (FISH) analysis of tissue slides. It also provides guidance for the creation of an IRB-approved study protocol, centralized pathology review of tissue submitted to the BLGSP, and enrollment and quality control forms. BLGSP SOPs are a product of the Office of Cancer Genomics (OCG) within the Center for Cancer Genomics of the National Cancer Institute (NCI) and are available from https://www.cancer.gov/ccg/research/structural-genomics/cgci/using-cgci-data/blgsp-sop-manual.pdf.