NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Office of Cancer Genomics (OCG) Burkitt Lymphoma Genome Sequencing Project (BLGSP) Standard Operating Procedures (SOP) Manual   Add to my SOP compendium

BRD ID: 2,201

Source: OCG Tier: SOP Number of times downloaded: 2554

Description

This document describes the procedures used by the Burkitt Lymphoma Genome Sequencing Project (BLGSP) for the collection, processing, preservation, and shipment of blood, buccal cell, and tissue specimens. Specific procedures included in the document include instruction for cell and tissue preservation by snap-freezing and/or formalin fixation; processing FFPE tissue scrolls; generating a peripheral blood smear; isolation of white blood cells and granulocytes with and without blood fractionation; creation of tissue microarrays; hematoxylin and eosin (H&E) staining, immunohistochemical staining, and fluorescence in situ hybridization (FISH) analysis of tissue slides. It also provides guidance for the creation of an IRB-approved study protocol, centralized pathology review of tissue submitted to the BLGSP, and enrollment and quality control forms. BLGSP SOPs are a product of the Office of Cancer Genomics (OCG) within the Center for Cancer Genomics of the National Cancer Institute (NCI) and are available from https://ocg.cancer.gov/resources/ocg-templates-and-protocols.

Documents for Download

BLGSP_SOP_manual.11.07.19.pdf [Size: 4579 KB]
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Biospecimens
  • Tissue - General
  • Tissue - Lymph Node
  • Fluid - Blood
  • Cell - Buccal
  • Cell - White Cells
Diagnoses
  • Neoplastic - Lymphoma
Topics
  • Preaquisition
  • Biospecimen Acquisition
  • Biospecimen Processing
  • Biospecimen Preservation
  • Biospecimen Shipment
  • Storage
  • Platform-specific Methodology

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