NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Office of Cancer Genomics (OCG) HIV+ Tumor Molecular Characterization Project (HTMCP) Standard Operating Procedures (SOP) Manual   Add to my SOP compendium

BRD ID: 2,122

Source: OCG Tier: SOP Number of times downloaded: 1758

Description

This document describes the procedures used by the HIV+ Tumor Molecular Characterization Project (HTMCP; https://www.cancer.gov/ccg/research/genome-sequencing/cgci/about/projects#hiv-tumor-molecular-characterization-project-htmcp) for the collection, processing, preservation, and shipment of blood, buccal cell, and tissue specimens. Specific procedures included in the document include instruction for cell and tissue preservation by snap-freezing and formalin fixation; processing FFPE tissue scrolls; tissue sectioning for hematoxylin and eosin (H&E) staining and macromolecule extraction; isolation of white blood cells via blood fractionation; sample storage at Tissue Source Sites (TSSs); and shipment of samples to the Genome Science Center at British Columbia (GSC-BC). It also provides guidance for creation of an IRB-approved study protocol and informed consent forms, allocation of sample IDs, sample labeling and documentation, and the centralized pathology review of tissue and sample submission for the HIV+ Diffuse Large B-Cell Lymphoma Characterization Project, HIV+ Lung Tumor Characterization Project, and the HIV+ Cervical Tumor Characterization Project. HTMCP SOPs are a product of the Office of Cancer Genomics (OCG) within the Center for Cancer Genomics of the National Cancer Institute (NCI) and are available from https://www.cancer.gov/ccg/research/structural-genomics/cgci/using-cgci-data/htmcp-sop-manual.pdf.

Documents for Download

HTMCP_SOP_manual.pdf [Size: 6604 KB]

Biospecimens
  • Cell - Buccal
  • Cell - White Cells
  • Fluid - Blood
  • Tissue - General
  • Tissue - Cervix
  • Tissue - Lung
  • Tissue - Lymph Node
Diagnoses
  • AIDS/HIV-related
Topics
  • Preaquisition
  • Biospecimen Acquisition
  • Biospecimen Processing
  • Biospecimen Preservation
  • Biospecimen Shipment
  • Storage
  • Platform-specific Methodology

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