NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Office of Cancer Genomics (OCG) HIV+ Tumor Molecular Characterization Project (HTMCP) Standard Operating Procedures (SOP) Manual   Add to my SOP compendium

BRD ID: 2,122

Source: OCG Tier: SOP Number of times downloaded: 1600

Description

This document describes the procedures used by the HIV+ Tumor Molecular Characterization Project (HTMCP) for the collection, processing, preservation, and shipment of blood, buccal cell, and tissue specimens. Specific procedures included in the document include instruction for cell and tissue preservation by snap-freezing and formalin fixation; processing FFPE tissue scrolls; tissue sectioning for hematoxylin and eosin (H&E) staining and macromolecule extraction; isolation of white blood cells via blood fractionation; sample storage at Tissue Source Sites (TSSs); and shipment of samples to the Genome Science Center at British Columbia (GSC-BC). It also provides guidance for creation of an IRB-approved study protocol and informed consent forms, allocation of sample IDs, sample labeling and documentation, and the centralized pathology review of tissue and sample submission for the HIV+ Diffuse Large B-Cell Lymphoma Characterization Project, HIV+ Lung Tumor Characterization Project, and the HIV+ Cervical Tumor Characterization Project. HTMCP SOPs are a product of the Office of Cancer Genomics (OCG) within the Center for Cancer Genomics of the National Cancer Institute (NCI) and are available from https://ocg.cancer.gov/resources/ocg-templates-and-protocols.

Documents for Download

HTMCP_SOP_manual.pdf [Size: 6604 KB]

Biospecimens
  • Cell - Buccal
  • Cell - White Cells
  • Fluid - Blood
  • Tissue - General
  • Tissue - Cervix
  • Tissue - Lung
  • Tissue - Lymph Node
Diagnoses
  • AIDS/HIV-related
Topics
  • Preaquisition
  • Biospecimen Acquisition
  • Biospecimen Processing
  • Biospecimen Preservation
  • Biospecimen Shipment
  • Storage
  • Platform-specific Methodology

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