Office of Cancer Genomics (OCG) HIV+ Tumor Molecular Characterization Project (HTMCP) Standard Operating Procedures (SOP) Manual
BRD ID: 2,122
Source: OCG Tier: SOP Number of times downloaded: 1758
Description
This document describes the procedures used by the HIV+ Tumor Molecular Characterization Project (HTMCP; https://www.cancer.gov/ccg/research/genome-sequencing/cgci/about/projects#hiv-tumor-molecular-characterization-project-htmcp) for the collection, processing, preservation, and shipment of blood, buccal cell, and tissue specimens. Specific procedures included in the document include instruction for cell and tissue preservation by snap-freezing and formalin fixation; processing FFPE tissue scrolls; tissue sectioning for hematoxylin and eosin (H&E) staining and macromolecule extraction; isolation of white blood cells via blood fractionation; sample storage at Tissue Source Sites (TSSs); and shipment of samples to the Genome Science Center at British Columbia (GSC-BC). It also provides guidance for creation of an IRB-approved study protocol and informed consent forms, allocation of sample IDs, sample labeling and documentation, and the centralized pathology review of tissue and sample submission for the HIV+ Diffuse Large B-Cell Lymphoma Characterization Project, HIV+ Lung Tumor Characterization Project, and the HIV+ Cervical Tumor Characterization Project. HTMCP SOPs are a product of the Office of Cancer Genomics (OCG) within the Center for Cancer Genomics of the National Cancer Institute (NCI) and are available from https://www.cancer.gov/ccg/research/structural-genomics/cgci/using-cgci-data/htmcp-sop-manual.pdf.
Documents for Download
HTMCP_SOP_manual.pdf [Size: 6604 KB]Biospecimens
- Cell - Buccal
- Cell - White Cells
- Fluid - Blood
- Tissue - General
- Tissue - Cervix
- Tissue - Lung
- Tissue - Lymph Node
Diagnoses
- AIDS/HIV-related
Topics
- Preaquisition
- Biospecimen Acquisition
- Biospecimen Processing
- Biospecimen Preservation
- Biospecimen Shipment
- Storage
- Platform-specific Methodology
Associated SOP Compendiums
- Preaquisition
- Biospecimen Acquisition
- Biospecimen Processing
- Biospecimen Preservation
- Biospecimen Shipment
- Storage
- Platform-specific Methodology