Influence of tissue preparation on the acoustic properties of tissue sections at high frequencies.
Author(s): Sasaki H, Saijo Y, Tanaka M, Nitta S
Publication: Ultrasound Med Biol, 2003, Vol. 29, Page 1367-72
PubMed ID: 14553815 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of freezing formalin-fixed renal tissue rather than embedding fixed specimens in paraffin on scanning acoustic microscopic analysis. After formalin fixation, specimens were either embedded in paraffin or frozen in acetone.
Summary of Findings:
Fat-containing renal cancer specimens (clear cell and granular cell) had significantly higher attenuation constants in formalin-fixed frozen sections than in formalin-fixed paraffin-embedded sections. There were no differences in attenuation constants between frozen and paraffin-embedded sections of fat-free oncocytoma specimens, nor were there any significant differences in sound speeds between frozen and paraffin-embedded sections for any of the tumor subtypes examined. Clear cell renal cancer specimens had significantly higher attenuation constants than granular cell cancer specimens among the frozen sections, and frozen sections of oncocytoma specimens had significantly lower attenuation constants than frozen sections of granular cell cancer specimens. No other acoustic property differences were observed between similarly preserved (frozen or paraffin-emebdded) tumor subtypes.
Biospecimens
Preservative Types
- Formalin
Diagnoses:
- Neoplastic - Carcinoma
- Neoplastic - Benign
Platform:
Analyte Technology Platform Morphology H-and-E microscopy Morphology Electron microscopy Morphology Scanning acoustic microscopy Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Preservation Embedding medium Conventional paraffin embedding
Not embedded
Preaquisition Diagnosis/ patient condition Clear cell renal cancer
Granular cell renal cancer