Influence of tissue preparation on the high-frequency acoustic properties of normal kidney tissue.

Author(s): Sasaki H, Saijo Y, Tanaka M, Okawai H, Terasawa Y, Yambe T, Nitta S

Publication: Ultrasound Med Biol, 1996, Vol. 22, Page 1261-5

PubMed ID: 9123651 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to compare the retention of acoustic properties in frozen, FFPE, and FFF normal kidney specimens. The latter preparation method consisted of formalin fixation and subsequent freezing of tissue in acetone instead of embedding it in paraffin.

   Summary of Findings:

   The renal corpuscle and renal tubules were not distinguishable in frozen sections of unfixed kidney tissue, while they were in FFPE and FFF preparations. The attenuation constants and sound speeds of the whole renal cortex or the renal medulla were not significantly different between preparation methods.

   Biospecimens

   • Tissue - Kidney

   Preservative Types

   • Formalin
   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Morphology	Scanning acoustic microscopy

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Formalin (buffered) Frozen
   Biospecimen Preservation	Embedding medium	Conventional paraffin embedding Not embedded
   Biospecimen Aliquots and Components	Biospecimen components	Renal corpuscle Renal tubule Renal cortex Renal medulla

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