Assessment of cell viability, early apoptosis, and hematopoietic potential in umbilical cord blood units after storage.
Author(s): Kim KM, Huh JY, Hong SS, Kang MS
Publication: Transfusion, 2015, Vol. 55(8), Page 2017-22
PubMed ID: 25858170 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effect of 8 years of frozen storage on umbilical cord blood (UCB) cell viability and apoptosis.
Conclusion of Paper
94.8% of total nucleated cells (TNCs) and 57.5% of CD34 positive cells were recovered from thawed UCB that had been frozen for 8 years. Although TNC viability was higher post-thaw in this study than found in a previous study for specimens prior to freezing, mononuclear cells (MNC) and CD34 positive cell viability were comparable between the studies. The percentage of early apoptotic (7-AAD negative and Annexin V positive) TNCs, MNC and CD34 positive cells were comparable in UCB pre-freeze and post-thaw.
Studies
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Study Purpose
The purpose of this study was to determine the effects of 8 years of frozen storage on UCB cell viability, and apoptosis. Cord blood was shipped at room temperature within 24 h of collection and analyzed. Subsequently, specimens were volume-reduced, red blood cell-depleted and frozen in dimethyl sulfoxide (DMSO) and dextran-40 in liquid nitrogen within 36 h of collection. Specimens were thawed at 37°C. Cell viability results were compared to those in 30 fresh UCB specimens analyzed within 48 h of collection.
Summary of Findings:
Post-thaw recoveries of 94.8% and 57.5% of pre-freeze levels were found for TNCs and CD34 positive cells, respectively (p<0.001, both). TNC viability, as assessed by 7-AAD and 7-AAD/annexin V, was higher post-thaw than found in a previous study prior to freezing (p<0.001, p=0.017), but MNC and CD34 positive cell viability were not different. The percentage of TNCs, MNC and CD34 positive cells expressing early apoptotic markers were comparable in UCB pre-freeze and post-thaw.
Biospecimens
Preservative Types
- None (Fresh)
- Frozen
Diagnoses:
- Pregnant
Platform:
Analyte Technology Platform Cell count/volume Hematology/ auto analyzer Cell count/volume Flow cytometry Cell count/volume Light microscopy Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Freeze/thaw cycling 0 cycles
1 cycle
Biospecimen Preservation Type of fixation/preservation Frozen
None (fresh)