Red blood cell storage increases hypoxia-induced nitric oxide bioavailability and methemoglobin formation in vitro and in vivo.
Author(s): Almac E, Bezemer R, Hilarius-Stokman PM, Goedhart P, de Korte D, Verhoeven AJ, Ince C
Publication: Transfusion, 2014, Vol. 54(12), Page 3178-85
PubMed ID: 24942042 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects of refrigerated storage of red blood cells (RBC) on nitric oxide (NO) and methemoglobin formation in response to nitrite under deoxygenated and oxygenated conditions.
Conclusion of Paper
Under deoxygenated conditions, RBC refrigerated for 5-6 weeks had higher methemoglobin and NO formation 10 min after nitrite administration than those refrigerated for <1 week, but no difference was noted under normal oxygenation or after washing off the nitrite.
Studies
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Study Purpose
The purpose of this study was to determine the effects of refrigerated storage of RBC on NO and methemoglobin formation in response to nitrite under deoxygenated and oxygenated conditions. Leukoreduced RBC from 6 patients were stored in saline-adenine-glucose-mannitol (SAGM) at 2-6 degrees C for <1 week or 5-6 weeks before being suspended in saline/glucose/Tris/sucrose/HCO3- and incubated at 37 degrees C. Cells were deoxygenated and sodium nitrite was added before measurement of NO for 10 min followed by methemoglobin levels.
Summary of Findings:
Under deoxygenated conditions, RBC refrigerated for 5-6 weeks had higher methemoglobin and NO formation 10 min after nitrite administration than RBCs refrigerated for <1 week (p=0.022 and p=0.026, respectively), but no difference was noted under normal oxygenation or after washing off the nitrite.
Biospecimens
Preservative Types
- Other Preservative
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Gas Chemiluminescence Protein Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration <1 week
5-6 weeks
Biospecimen Aliquots and Components Biospecimen components 50% oxygenated hemoglobin
100% oxygenated hemoglobin
Analyte Extraction and Purification Washing 250 umol/L sodium nitrite
No sodium nitrite