NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Randomized study of washing 40- to 42-day-stored red blood cells.

Author(s): Bennett-Guerrero E, Kirby BS, Zhu H, Herman AE, Bandarenko N, McMahon TJ

Publication: Transfusion, 2014, Vol. 54(10), Page 1362544-52

PubMed ID: 24735194 PubMed Review Paper? No

Purpose of Paper

The purpose of this paper was to determine the effects of washing method on markers of red blood cell (RBC) quality and RBC fragility in RBCs refrigerated for 40-42 days.

Conclusion of Paper

Compared to unwashed RBCs, washing RBCs with the Cell Saver Elite decreased hematocrit (Hct), glucose, lactate, potassium and CD235 negative microparticles and increased volume, cell free volume, hemoglobin saturation, hemolysis, total free hemoglobin, RBC microparticles and CD235 positive microparticles, but washing with the COBE 2991 only decreased CD235 negative microparticles. Comparing the two washing methods, specimens washed with the COBE 2991 had higher Hct, total free hemoglobin, lactate and potassium and lower glucose levels, hemoglobin saturation, hemolysis, volume, RBC microparticles, and cell free volume than those washed with Cell Saver Elite, but RBC recovery was not affected by the method of washing. after ATP levels and susceptibility to stress-induced hemolysis (agitation or filtration) were not significantly affected by washing or method of washing.

Studies

  1. Study Purpose

    The purpose of this study was to determine the effects of washing method on markers of RBC quality and RBC fragility in RBCs refrigerated for 40-42 days. 40 RBC units stored 40-42 days were obtained from the American Red Cross and analyzed immediately, and then 20 units each were washed with the COBE2991 or the Cell Saver Elite.

    Summary of Findings:

    Compared to unwashed RBCs, washing with the Cell Saver Elite decreased Hct (p<0.05), glucose (p<0.05), lactate (p<0.05), potassium (p<0.05), and CD235 negative microparticles and increased volume (P<0.05), cell free volume (P<0.05), hemoglobin saturation (p<0.05), hemolysis (p<0.05), total free hemoglobin (p<0.05), CD235 positive microparticles (p<0.05) and RBC microparticles, but washing only reduced the number of CD235 negative microparticles (p<0.05). Comparing the two washing methods, specimens washed with the COBE 2991 had higher Hct (p<0.05), total free hemoglobin (p<0.05), lactate (p<0.05) and potassium (p<0.05) and lower glucose levels (p<0.05), hemoglobin saturation (p<0.05), hemolysis (p<0.05), volume (p<0.05), RBC microparticles (p<0.05), CD235 positive microparticles (p<0.05) and cell free volume (p<0.05) than specimens washed with the Cell Saver Elite, but RBC recovery was not affected by the method of washing. ATP levels and susceptibility to stress-induced hemolysis (agitation or filtration) were not significantly affected by washing or method of washing.

    Biospecimens
    Preservative Types
    • Other Preservative
    Diagnoses:
    • Normal
    Platform:
    AnalyteTechnology Platform
    Small molecule Enzyme assay
    Small molecule Clinical chemistry/auto analyzer
    Electrolyte/Metal Clinical chemistry/auto analyzer
    Protein Spectrophotometry
    Cell count/volume Spectrophotometry
    Cell count/volume Flow cytometry
    Carbohydrate Clinical chemistry/auto analyzer
    Pre-analytical Factors:
    ClassificationPre-analytical FactorValue(s)
    Biospecimen Aliquots and Components Blood processing method Washed with COBE 2991
    Washed with Cell Saver Elite
    Unwashed
    Analyte Extraction and Purification Washing Unwashed
    Washed with Cell Saver Elite
    Washed with COBE 2991

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