Stability of relevant plasma protein activities in cryosupernatant plasma units during refrigerated storage for up to 5 days postthaw.
Author(s): Bhakta V, Jenkins C, Ramirez-Arcos S, Sheffield WP
Publication: Transfusion, 2013, Vol. 54(2), Page 418-25
PubMed ID: 23692473 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to compare cryosupernatant plasma (CSP) with fresh or frozen plasma and to determine the effects of post-thaw storage of CSP on coagulation factors.
Conclusion of Paper
Compared to published values for fresh plasma, CSP had lower levels of Factor (F)V, FVIII, and fibrinogen and a longer prothrombin time (PT). Post-thaw refrigerated storage of CSP led to declines in FVII and FV levels and elongation of PT after 1 day of storage, and A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS13) activity was lower after 5 days of refrigerated storage than 1 h after CSP specimens were thawed. Compared with frozen plasma, CSP lacked the large von Willebrand Factor (vWF) multimers, but post-thaw storage had no effect on vWF levels or vWF multimer distribution, and factor VIII levels also remained relatively constant during post-thaw storage of CSP.
Studies
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Study Purpose
The purpose of this study was to compare CSP with fresh or frozen plasma and to determine the effects of post-thaw storage on coagulation factors in CSP. At three different collection sites within Canada, plasma was obtained by filtration of whole blood. Plasma was frozen and slowly thawed to obtain cryosupernatant plasma (CSP). CSP was shipped on dry ice to the testing facility and stored at -80 degrees C for 10-11 months. CSP was thawed in a shaking water bath at 37 degrees C for 20 min and then transferred to 2-8 degrees C. Values were compared with published values in fresh plasma and frozen plasma. Some significant differences between the CSP used in this study and CSP used in four other publications were identified.
Summary of Findings:
CSP levels of FV, FVIII, and fibrinogen were 76%, 16%, and 60%, respectively, of those reported in fresh plasma (P<0.0001, all), and the prothrombin time (PT) of CSP was, on average, 1.3 seconds longer than published values for fresh plasma (P<0.0001). Post-thaw refrigerated storage of CSP led to declines in FVII and FV levels after 1 day (3.1% and p<0.05 and 10.2% and p<0.001, respectively) and small additional declines with longer storage. ADAMTS13 was 2.6% lower after 5 days of post-thaw refrigerated storage than levels measured 1 h after thawing CSP (p<0.05). PT increased consistently by 0.15-0.20 seconds per day of post-thaw refrigerated storage of CSP with significant increases observed after just 1 day (p<0.001). Compared with frozen plasma, CSP lacked the large vWF multimers, but post-thaw storage had no effect on vWF levels or vWF multimer distribution, and factor VIII levels also remained relatively constant during post-thaw storage of CSP.
Biospecimens
Preservative Types
- Frozen
- None (Fresh)
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Protein ELISA Protein Clinical chemistry/auto analyzer Protein 1D/2D gels Protein Hematology/ auto analyzer Glycoprotein Hematology/ auto analyzer Glycoprotein ELISA Morphology Hematology/ auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Preservation Type of fixation/preservation None (fresh)
Frozen
Storage Storage duration 1 h
1 day
2 days
5 days
Biospecimen Aliquots and Components Blood and blood products Cryosupernatant plasma
Plasma