Collection and storage of red blood cells with anticoagulant and additive solution with a physiologic pH

Author(s): Burger P, Korsten H, Verhoeven AJ, de Korte D, van Bruggen R

Publication: Transfusion, 2012, Vol. 52, Page 1245

PubMed ID: 22233266 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of type and pH of anticoagulant and pH of storage solution used for preparation of leukoreduced RBC during cold storage on hematological and clinical chemistry analytes. Whole blood specimens were stored for 12 to 18 h at 22 to 24 degrees C before leukoreduction by centrifugation and removal of the buffy coat. Additive solution was added and RBC were filtered to remove any residual leukocytes. Specimens were then stored in polyvinylchloride storage bags at 2 to 6 degrees C. After experimental refrigerated storage, aliquots were prepared for 2,3-DPG, ATP, and lactate measurement and frozen until analysis.

   Summary of Findings:

   When blood was collected into TNC (pH 7.4), intracellular and extracellular pH, potassium levels, and 2,3 DPG levels were significantly higher during the first two weeks of refrigerated storage of leukoreduced RBC in SAGM (pH 6.2) compared to levels in specimens collected in CPD (pH 5.6). Hemolysis, glucose, lactate, and ATP levels were not significantly affected by the type of anticoagulant, although levels of glucose and lactate decreased and increased, respectively throughout the 5 weeks of refrigerated storage in both TNC and CPD specimens. When leukoreduced RBC were prepared from TNC whole blood and either PAGGGM with a pH of 7.4 or 8.2 was added, there were no differences in intracellular pH, extracellular pH, potassium, hemolysis, glucose, lactate, ATP, or 2,3-DPG levels between the two additive solutions during refrigerated storage. However, intracellular and extracellular pH and glucose decreased during refrigerated storage of leukoreduced RBC, and potassium and lactate increased. A comparison of SAGM (pH 6.2) and PAGGGM (pH 7.4 and 8.2) units from TNC whole blood revealed that while intracellular and extracellular pH and hemolysis were generally similar, the rest of the analytes showed significant differences between the additive solutions at some point during the 5 weeks of refrigerated storage.

   Biospecimens

   • Cell - Red Blood Cells

   Preservative Types

   • Other Preservative
   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Small molecule	Clinical chemistry/auto analyzer
   Protein	Spectrophotometry
   Electrolyte/Metal	Clinical chemistry/auto analyzer
   Carbohydrate	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Frozen Refrigeration
   Storage	Storage duration	0 d 7 d 14 d 21 d 28 d 35 d
   Storage	Short-term storage solution	SAGM pH 6.2 PAGGGM pH 7.4 PAGGGM pH 8.2
   Biospecimen Aliquots and Components	pH	5.6 6.2 7.4 8.2
   Biospecimen Acquisition	Anticoagulant	Citrate-phosphate-dextrose Sodium citrate

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