Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Activity of clotting factors in fresh-frozen plasma during storage at 4 degrees C over 6 days.

Author(s): von Heymann C, Keller MK, Spies C, Schuster M, Meinck K, Sander M, Wernecke KD, Kiesewetter H, Pruss A

Publication: Transfusion, 2009, Vol. 49, Page 913-20

PubMed ID: 19159416 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of freezing and post-thaw refrigerated storage of plasma on clotting factor activity. Sodium citrate plasma from 20 healthy donors was stored frozen at -30°C for at least 8 weeks before thawing in a Plasmatherm III. Thawed plasma was stored at 4°C.

   Summary of Findings:

   Compared to fresh plasma, plasma that had previously been frozen had lower levels of fibrinogen, FII, FV, FVII, FX, FXIII, PC and ATIII (p<0.001, all).  FII, FV, FVII, FVIII, FIX, FX, FXI and FPS decreased significantly between 0 h and 6 days of post-thaw refrigerated storage  (p<0.001, all). Further, compared to levels of activity measured immediately after thawing, previously frozen plasma stored for 6 days had higher FXII (p=0.023), FXIII (p<0.001) and PC (p=0.003). Levels of von Willebrand factor antigen (VWF-Ag) and FPS were not affected by freezing or post-thaw refrigerated storage of plasma.

   Biospecimens

   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)
   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	Hematology/ auto analyzer
   Glycoprotein	Hematology/ auto analyzer
   Protein	Immunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	None (fresh) Frozen
   Storage	Storage duration	0 h 6 days

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