Overnight storage of whole blood: a comparison of two designs of butane-1,4-diol cooling plates.
Author(s): Van der Meer PF, Pietersz RN
Publication: Transfusion, 2007, Vol. 47, Page 2038-43
PubMed ID: 17958532 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of the rate at which whole blood reaches room temperature on markers of RBC quality during refrigerated storage. Donor blood was cooled to room temperature on Compocool or CompoCool WB plates, and once cool (<2 h), an aliquot was taken, and the remainder was left on the bench overnight at room temperature before blood component processing. RBCs were then stored at 4 degrees C and periodically sampled.
Summary of Findings:
After overnight room temperature storage, whole blood cooled on CompoCool WB and subsequently isolated RBCs from that whole blood had higher ATP levels than RBCs from whole blood cooled on Compocool. When whole blood was stored overnight at room temperature, rather than analyzed within 2 h, ATP and Factor VIII levels declined, regardless of cooling method, and platelet counts increased in the Compocool-cooled specimens only. Hemoglobin levels, hematocrit, white blood cell counts, platelet counts, volume and hemolysis rates of plasma, buffy coat or RBCs were not affected by room temperature storage of whole blood or specimen cooling method. During storage of RBCs at 4 degrees C, the hemolysis rate, PO2, PCO2, and lactate increased, and pH, bicarbonate, glucose and ATP levels decreased. Among stored RBCs, only ATP levels on day 1 and lactate levels on day 35 were significantly different between specimens cooled on Compocool and those cooled on CompoCool WB plates.
Biospecimens
Preservative Types
- None (Fresh)
- Other Preservative
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Carbohydrate Clinical chemistry/auto analyzer Cell count/volume Hematology/ auto analyzer Gas Clinical chemistry/auto analyzer Protein Clinical chemistry/auto analyzer Protein Hematology/ auto analyzer Small molecule Clinical chemistry/auto analyzer Small molecule Spectrophotometry Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 1 day
28 days
35 days
42 days
Biospecimen Preservation Type of fixation/preservation None (fresh)
Refrigeration
Storage Time at room temperature <2 h
16-20 h
Biospecimen Preservation Cooling or freezing method/ rate CompoCool