Overnight storage of whole blood: a comparison of two designs of butane-1,4-diol cooling plates.

Author(s): Van der Meer PF, Pietersz RN

Publication: Transfusion, 2007, Vol. 47, Page 2038-43

PubMed ID: 17958532 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of the rate at which whole blood reaches room temperature on markers of RBC quality during refrigerated storage. Donor blood was cooled to room temperature on Compocool or CompoCool WB plates, and once cool (<2 h), an aliquot was taken, and the remainder was left on the bench overnight at room temperature before blood component processing. RBCs were then stored at 4 degrees C and periodically sampled.

   Summary of Findings:

   After overnight room temperature storage, whole blood cooled on CompoCool WB and subsequently isolated RBCs from that whole blood had higher ATP levels than RBCs from whole blood cooled on Compocool. When whole blood was stored overnight at room temperature, rather than analyzed within 2 h, ATP and Factor VIII levels declined, regardless of cooling method, and platelet counts increased in the Compocool-cooled specimens only. Hemoglobin levels, hematocrit, white blood cell counts, platelet counts, volume and hemolysis rates of plasma, buffy coat or RBCs were not affected by room temperature storage of whole blood or specimen cooling method. During storage of RBCs at 4 degrees C, the hemolysis rate, PO2, PCO2, and lactate increased, and pH, bicarbonate, glucose and ATP levels decreased. Among stored RBCs, only ATP levels on day 1 and lactate levels on day 35 were significantly different between specimens cooled on Compocool and those cooled on CompoCool WB plates.

   Biospecimens

   • Bodily Fluid - Blood
   • Cell - Red Blood Cells

   Preservative Types

   • None (Fresh)
   • Other Preservative

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Carbohydrate	Clinical chemistry/auto analyzer
   Cell count/volume	Hematology/ auto analyzer
   Gas	Clinical chemistry/auto analyzer
   Protein	Clinical chemistry/auto analyzer
   Protein	Hematology/ auto analyzer
   Small molecule	Clinical chemistry/auto analyzer
   Small molecule	Spectrophotometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	1 day 28 days 35 days 42 days
   Biospecimen Preservation	Type of fixation/preservation	None (fresh) Refrigeration
   Storage	Time at room temperature	<2 h 16-20 h
   Biospecimen Preservation	Cooling or freezing method/ rate	CompoCool

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