Stability of platelet surface antigens during storage.

Author(s): Dunstan RA, Simpson MB

Publication: Transfusion, 1985, Vol. 25, Page 563-6

PubMed ID: 4071601 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the stability of platelet surface antigens including A, B, 2H, PI(A1), and HLA class I antigens after storage of up to 10 days at 22 degrees C and after storage for 24 h in one of the following types of media: 20% acid-citrate-dextrose in 60% lyposin and 20% albumin, pH 5.8; 20% acid-citrate-dextrose in 40% lyposin and 40% albumin, pH 6.0; 20% acid-citrate-dextrose in 20% lyposin and 60% albumin, pH 6.1; 20% acid-citrate-dextrose in 8% lyposin, pH 5.3; 20% acid-citrate-dextrose in 20% normal serum albumin, pH 6.2.

   Summary of Findings:

   No significant changes were seen in any of the tested antigens after 10 days of storage at 22 degrees C (p>0.05) in citrate-phosphate-dextrose-adenine. Furthermore, storage for 24 h in different types of media had no significant impact on platelet antigen levels with the exception of an approximately 50% decrease in A antigen on platelets suspended in homologous O plasma, 20% acid-citrate-dextrose in 20% normal serum albumin pH 6.2, or 20% acid-citrate-dextrose in 20% lyposin and 60% albumin pH 6.1.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified
   • Normal

   Platform:

   Analyte	Technology Platform
   Small molecule	Radioimmunoassay
   Cell count/volume	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	1 d 3 d 5 d 10 d
   Biospecimen Preservation	Fixative additive/buffer	Albumin Autologous plasma Homologous plasma Lyposin Multiple concentrations evaluated None Normal serum albumin PBS-EDTA pH 7.2
   Storage	Short-term storage solution	20% acid-citrate-dextrose in 60% lyposin and 20% albumin, pH 5.8 20% acid-citrate-dextrose in 40% lyposin and 40% albumin, pH 6.0 20% acid-citrate-dextrose in 20% lyposin and 60% albumin, pH 6.1 20% acid-citrate-dextrose in 8% lyposin, pH 5.3 20% acid-citrate-dextrose in 20% normal serum albumin, pH 6.2

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