Red blood cell storage and cell morphology.

Author(s): Blasi B, D'Alessandro A, Ramundo N, Zolla L

Publication: Transfus Med, 2012, Vol. 22, Page 90-6

PubMed ID: 22394111 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage duration at 4 degrees C on pH, potassium levels, hemolysis, osmotic fragility and ESR of donated RBCs in SAG-M. Whole blood from 8 healthy donors was anticoagulated with citrate phosphate dextrose adenine (CPDA), and RBCs were obtained by centrifugation following standard blood bank conditions.

   Summary of Findings:

   The average pH of the internal RBCs and the supernatant declined linearly from 6.9 and 7.0, respectively, to 6.45 and 6.55, respectively, during the 42 days of storage. The average supernatant potassium concentrations increased from 2.5 mmol/L to 27.5 mmol/L during the 42 days of storage, with a faster rate of increase observed in the first 14 days than between days 15-42. Similarly, the rate of hemolysis increased during storage but remained below the 0.8% threshold for medical use. The osmotic fragility increased with increasing storage beyond day 14. Further, with progressive storage, the ESR increased linearly from 2-4.5 mm/h to 5.5-6.5 mm/h in specimens from each of the subjects. With increasing storage, the percentage of RBCs with a discocyte morphology decreased from 76.2% at day 7 to 22.9% at day 42, and the percentage of cells showing irreversible morphological changes increased from 8.8% to 39.5%, with more than 25% displaying irreversible changes starting after day 28, and more than 25% displaying a non-discocyte morphology after 21 days.

   Biospecimens

   • Cell - Red Blood Cells

   Preservative Types

   • None (Fresh)
   • Other Preservative

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Electrolyte/Metal	Ion selective electrode
   Small molecule	pH
   Cell count/volume	Spectrophotometry
   Morphology	Electron microscopy

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	0 days 7 days 14 days 21 days 28 days 35 days 42 days
   Biospecimen Preservation	Type of fixation/preservation	None (fresh) Refrigeration

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