Effect of freeze-drying, freezing and frozen storage of blood plasma on fibrin network characteristics.
Author(s): Pieters M, Jerling JC, Weisel JW
Publication: Thromb Res, 2002, Vol. 107, Page 263-9
PubMed ID: 12479888 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
-
Study Purpose
The purpose of this study was to determine the effects of freeze-drying or freezing a single plasma pool on fibrin content, permeability, compaction of the clot, fiber mass to length ratio, and fibrinogen content. Pooled plasma was stored freeze-dried or refrigerated at 4 degrees C or was stored frozen at -83 degrees C for 12 h and then reconstituted or thawed at 25 degrees C as necessary.
Summary of Findings:
When freeze-dried plasma was reconstituted, the mass-length ratio of the fibers, the compaction and fibrin content of the clots, and the fibrinogen content were the same as in refrigerated plasma, but the permeability of the clots was lower than in refrigerated plasma (P=0.005). Frozen plasma had significantly higher mass-length ratios of the fibers (p<0.001), and compaction of the clots (p<0.001) and significantly lower fibrin (p=0.002) and fibrinogen content (p=0.04), but similar permeability, compared to that observed in refrigerated plasma.
Biospecimens
Preservative Types
- Frozen
- Other Preservative
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Protein Hematology/ auto analyzer Glycoprotein Hematology/ auto analyzer Morphology Macroscopic observation Morphology Hematology/ auto analyzer Morphology Spectrophotometry Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Preservation Type of fixation/preservation Frozen
Refrigeration
Lyophilized
-
Study Purpose
The purpose of this study was to determine the effects of refrigerated storage of freeze-dried plasma and frozen storage of reconstituted freeze-dried plasma on fibrin content, permeability, compaction of the clot, fiber mass to length ratio, and fibrinogen content. Aliquots of a single freeze-dried plasma pool were stored at 4 degrees C, between 12 h and 4 months, prior to reconstitution. Reconstituted, freeze-dried plasma was then stored frozen at -83 degrees C for the remaining time for a combined storage of 4 months. The first aliquot (12 h) and the last aliquot (4 months) were reconstituted on the day of analysis and were not frozen. All stored aliquots were analyzed together to eliminate the effect of analytical variability.
Summary of Findings:
Refrigerated storage of freeze-dried plasma for 4 months led to decreased permeability (-23%, p<0.05) and fibrin content of the clot (-30%, p<0.05) and increased fibrinogen (9.8%, p<0.05) compared to freeze-dried plasma analyzed after 12 h of refrigerated storage, but the decrease in permeability was less than the critical difference (40.83) indicating this may be due to analytical variation. With increasing duration of frozen storage of reconstituted plasma (corresponding decreased duration of refrigerated storage of freeze-dried plasma), there were decreases in the mass-length ratios of the fibers (r=-0.66, p<0.001) and the compaction of the clots (r=-0.42, p=0.011) and increases in fibrin content of the clots (r=0.33, p=0.049). Frozen storage duration had no effects on the permeability of the clot or fibrinogen content.
Biospecimens
Preservative Types
- Other Preservative
- Frozen
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Morphology Hematology/ auto analyzer Morphology Macroscopic observation Morphology Spectrophotometry Protein Hematology/ auto analyzer Glycoprotein Hematology/ auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Preservation Type of fixation/preservation Frozen
Lyophilized
Storage Storage conditions Freeze-dried plasma
Reconstituted plasma
Storage Storage temperature 4 degrees C
-83 degrees C
Storage Storage duration 12 h
1 month
2 months
3 months
4 months