Longitudinal stability of coagulation, fibrinolysis, and inflammation factors in stored plasma samples.

Author(s): Lewis MR, Callas PW, Jenny NS, Tracy RP

Publication: Thromb Haemost, 2001, Vol. 86, Page 1495-500

PubMed ID: 11776319 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of frozen storage at -70 degrees C on the levels of coagulation, fibrinolysis, and inflammation factors in frozen and lyophilized plasma pools. Frozen and lyophilized plasma pools were purchased from commercial vendors, and other plasma pools were produced in the laboratory. Experimental storage was considered to begin after first use in the laboratory.

   Summary of Findings:

   The mean coefficient of variance (CV) of the average monthly levels of the analytes ranged from 2.6% (fibrinogen)-11.1% (D-dimer). Effects of storage were observed in some individual frozen and lyophilized plasma pools. With increasing storage at -70 degrees C, 1 of 4 and 2 of 2 frozen plasma pools showed increasing CRP and PAI-1 levels, respectively, and 1 of 4 and 1 of 2 frozen plasma pools showed decreasing levels of CRP and Protein S, respectively. With increasing storage at -70 degrees C, 1 of 5, 1 of 2 and 1 of 1 lyophilized plasma pools showed increasing fibrinogen, PAI-I antigen and protein S levels, respectively, and 1 of 5 lyophilized plasma pools showed decreasing levels of fibrinogen. There was no significant effect of using lyophilized rather than frozen pools. Repeated measures analysis identified a significant storage by time effect on fibrinogen levels (p=0.013), but the authors report no trends were observed when the 4 month average levels were compared.

   Biospecimens

   • Bodily Fluid - Plasma

   Preservative Types

   • Frozen
   • Other Preservative

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	ELISA
   Protein	Clinical chemistry/auto analyzer
   Protein	Hematology/ auto analyzer
   Glycoprotein	Hematology/ auto analyzer
   Peptide	ELISA

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	7-59 months
   Biospecimen Preservation	Type of fixation/preservation	Frozen Lyophilized

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