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Plastic versus siliconized glass tubes: evaluation in current laboratory practice.

Author(s): Biron-Andréani C, Mallol C, Séguret F, Schved JF

Publication: Thromb Haemost, 2000, Vol. 83, Page 800-1

PubMed ID: 10823290 PubMed Review Paper? No

Purpose of Paper

The purpose of this paper was to compare coagulation analyses performed using specimens collected in siliconized glass or polyethylene terephtalate (plastic) tubes. Specimens were analyzed from healthy controls as well as patients receiving oral anticoagulants, unfractionated heparin, and low molecular weight heparin.

Conclusion of Paper

Some differences were observed between the two types of collection tubes, the most notable being a longer mean prothrombin time (PT) with the plastic tubes than with the siliconized glass tubes for specimens from healthy individuals (p=0.01) and patients receiving oral anticoagulants, unfractionated heparin, or low molecular weight heparin (p<0.005). Importantly, the same tubes should be used for obtaining reference values and performing coagulation analyses.

Studies

  1. Study Purpose

    The purpose of this study was to compare the results of several coagulation analyses between specimens collected in two different types of tubes. Analyses performed included activated partial thromboplastin time test (APTT), PT, fibrinogen, anti-Xa activity, factor XIIa (FXIIa) test, and prothrombin fragments 1 + 2 testing.

    Summary of Findings:

    No significant differences were seen with anti-Xa testing or APTT between the two types of tubes with the exception of APTT in patients receiving oral anti-coagulants. In this case, APTT was longer (49.2 s vs 48.5 s) in the siliconized glass tubes (p=0.01). Significant differences between the two collection tube types were also seen in PT and prothrombin fragments 1 + 2 testing for healthy individuals and those receiving oral anti-coagulants or heparin treatment, although the magnitude and direction of tube-mediated effect was dependent on patient diagnosis and the type of anti-coagulant administered. While average fibrinogen and FXIIa concentrations were significantly lower using the siliconized glass tubes for healthy individuals (p<0.005 and p=0.01), there was no significant difference for individuals taking oral anticoagulants.

    Biospecimens
    Preservative Types
    • None (Fresh)
    • Frozen
    Diagnoses:
    • Normal
    • Other diagnoses
    Platform:
    AnalyteTechnology Platform
    Glycoprotein Hematology/ auto analyzer
    Protein Hematology/ auto analyzer
    Pre-analytical Factors:
    ClassificationPre-analytical FactorValue(s)
    Biospecimen Preservation Type of fixation/preservation None (fresh)
    Snap frozen
    Storage Storage temperature -80 degrees C
    RT
    Biospecimen Acquisition Anticoagulant Citrate
    Biospecimen Acquisition Type of collection container/solution Siliconized glass tube
    Plastic tube
    Preaquisition Diagnosis/ patient condition Healthy
    Oral anticoagulant therapy
    Unfractionated heparin therapy
    Low molecular weight heparin therapy
    View more

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