A novel microscope slide adhesive for poorly adherent tissue sections
Author(s): Sompuram SR, McMahon D, Vani K, Ramanathan H, Bogen SA
Publication: The J Histotechnol, 2003, Vol. 26, Page 117-123
Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of slide adhesive on the retention of tissue sections after IHC and histological staining using several tissues which demonstrated poor slide adhesion in previous studies.
Summary of Findings:
While 31/107 tissue section pairs showed equivalent adhesion (graded from 1-3) on PI- and aminosilane-coated slides, 71/107 specimen pairs demonstrated superior adhesion with the PI-coated slides. The 5 pairs of sections where adhesion to aminosilane slides was superior to PI-coated slides had not undergone antigen retrieval. The authors suggest that wet heat associated with antigen retrieval may enhance the performance of PI-coated slides. PI-coated slides were also determined to be superior to poly-L-lysine- and polysine-coated slides. In some instances, the diagnostically important area of tissue was only evident on PI-coated slides and lost from the other slides. The authors did not note any differences between tissue types.
Biospecimens
- Tissue - Bone
- Tissue - Brain
- Tissue - Lymph Node
- Tissue - Breast
- Tissue - Bone Marrow
- Tissue - Muscle (Skeletal)
Preservative Types
- Formalin
Diagnoses:
- Not specified
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform Protein Immunohistochemistry Morphology Light microscopy Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Tissue section adhesion Protected isocyanate
Aminosilane
Poly-L-lysine
Polysine
Biospecimen Acquisition Biospecimen location Bone
Brain
Muscle
Breast
Bone marrow
Lymph node
Analyte Extraction and Purification Antigen retrieval None
Microwave in Antigen Retrieval Citra Plus
Microwave in EDTA