Stability of salivary steroids: the influences of storage, food and dental care.
Author(s): Gröschl M, Wagner R, Rauh M, Dörr HG
Publication: Steroids, 2001, Vol. 66, Page 737-41
PubMed ID: 11522335 PubMed Review Paper? No
Suggested by: ISBER
Purpose of Paper
The purpose of this paper was to determine the effects of diet, dental care, and storage parameters on salivary steroid levels.
Conclusion of Paper
Generally, food intake and dental care did not affect cortisol, 17-hydroxyprogesterone (17OHP), or progesterone (P) levels in saliva. When unprocessed saliva was stored for 21 days at room temperature or 4 degrees C, there were linear decreases in cortisol, 17OHP and P levels. Centrifugation of saliva prior to storage or the addition of trifluor acetate (TFA) attenuated the decreases in cortisol, 17OHP and P observed after storage. However, when saliva containing sodium azide (NaN3) was stored for 21 days at room temperature or 4 degrees C, only clinically insignificant declines in cortisol, 17OHP, and P levels were observed. Two freeze-thaw cycles rather than one had no effects on the concentrations of any analytes, but when saliva specimens were thawed more than twice, significant decreases in cortisol levels (10%), but not 17OHP or P levels were observed.
Studies
-
Study Purpose
The purpose of this study was to determine the effects of diet, dental care, preservation method, freeze-thaw cycling and storage on salivary steroid levels. After experimental storage, specimens were stored at -25 degrees C until analysis.
Summary of Findings:
Generally, food intake and dental care did not affect cortisol, 17OHP, or P levels in saliva, but P levels increased slightly after chewing a lemon slice (p<0.05). When unprocessed saliva was stored for 21 days at room temperature or 4 degrees C, there were linear decreases in cortisol, 17OHP and P levels, with clinical significance reached by day 5. Centrifugation of saliva prior to storage or the addition of TFA attenuated the decreases in cortisol, 17OHP and P observed after storage, but the effects of storage were still clinically relevant after 5-9. However, when saliva containing NaN3 was stored for 21 days at room temperature or 4 degrees C, only clinically insignificant declines in cortisol, 17OHP, and P levels were observed. Two freeze-thaw cycles rather than one had no effects on the concentrations of any analytes, but when saliva was thawed more than twice, significant decreases in cortisol levels (10%; p<0.001), but not 17OHP or P levels were observed.
Biospecimens
Preservative Types
- Frozen
- Other Preservative
Diagnoses:
- Normal
- Other diagnoses
Platform:
Analyte Technology Platform Steroid Radioimmunoassay Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Preaquisition Diagnosis/ patient condition Congenital adrenal hyperplasia
Biospecimen Acquisition Time of biospecimen collection Before eating
After eating bread
After eating a lemon slice
After drinking milk
Before teeth brushing
After teeth brushing
Storage Storage temperature 4 degrees C
Room temperature
Storage Storage duration 1 day
3 days
5 days
7 days
9 days
11 days
13 days
15 days
17 days
19 days
21 days
Storage Freeze/thaw cycling 1 cycle
2 cycles
3 cycles
4 cycles
5 cycles
Biospecimen Preservation Type of fixation/preservation None (fresh)
Sodium azide
Trifluor acetate
Biospecimen Aliquots and Components Centrifugation Centrifuged
Not centrifuged