Storage stability of dengue IgM and IgG antibodies in whole blood and serum dried on filter paper strips detected by ELISA.
Author(s): Ruangturakit S, Rojanasuphot S, Srijuggravanvong A, Duangchanda S, Nuangplee S, Igarashi A
Publication: Southeast Asian J Trop Med Public Health, 1994, Vol. 25, Page 560-4
PubMed ID: 7777926 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
This study investigated the stability of anti-dengue virus IgG and IgM antibodies in air-dried whole blood and serum specimens stored on filter paper at room temperature for 1-5 months in comparison to case-matched frozen serum controls stored at -20 degrees C.
Summary of Findings:
The immunoreactivity of air-dried specimens stored on filter paper at room temperature was adversely affected by storage duration, impacting both the number of primary dengue cases and percent agreement with case-matched frozen sera controls. While air-dried serum specimens had a greater percent agreement with frozen serum controls, decreases in the number of primary diagnosed cases as well as percent agreement occurred more rapidly compared to air-dried blood specimens, 3 months versus 6 months, respectively. The number of secondary cases of dengue remained unaffected by room temperature storage of air-dried specimens. The immuno-availability of anti-dengue IgM antibodies, in particular, seemed to be affected by filter paper preservation when analyzed by ELISA.
Biospecimens
Preservative Types
- Frozen
- Other Preservative
Diagnoses:
- Other diagnoses
Platform:
Analyte Technology Platform Protein ELISA Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Blood and blood products Serum
Whole blood
Storage Storage duration 1 month
3 months
4 months
5 months
Storage Storage temperature Room temperature
-20 degrees C
Biospecimen Preservation Type of fixation/preservation Air-dried
Frozen