Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Effect of repeated freeze-thaw cycles on urinary albumin-to-creatinine ratio.

Author(s): Bao Y, Zuo L

Publication: Scand J Clin Lab Invest, 2009, Vol. 69, Page 886-8

PubMed ID: 19929272 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   This study investigated the effects of freeze-thaw cycling on levels of albumin and creatinine in 53 urine specimens with abnormal albumin levels. Immediately after collection, specimens were placed in parafilm–sealed Eppendorf tubes and transported to the central laboratory on ice. Albumin and creatinine were first measured within 48h.  Specimens were analyzed again after each of five cycles of freezing at -30˚C and thawing for 1 h at room temperature.

   Summary of Findings:

   Up to five freeze-thaw cycles had no effect on the levels of urinary albumin or creatinine or the urinary albumin to creatinine ratio.

   Biospecimens

   • Bodily Fluid - Urine

   Preservative Types

   • None (Fresh)
   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Refrigeration Frozen
   Storage	Freeze/thaw cycling	0 cycles 1 cycle 2 cycles 3 cycles 4 cycles 5 cycles

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