Stability of albumin, protein HC, immunoglobulin G, kappa- and lambda-chain immunoreactivity, orosomucoid and alpha 1-antitrypsin in urine stored at various conditions.
Author(s): Tencer J, Thysell H, Andersson K, Grubb A
Publication: Scand J Clin Lab Invest, 1994, Vol. 54, Page 199-206
PubMed ID: 7518610 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to assess the stability of urinary proteins after storage at -20, 4, or 22 degrees C for varying durations in the presence or absence of a preservative.
Conclusion of Paper
Of the seven urinary proteins examined, alpha1-antitrypsin alone decreased after storage at 22 or 4 degrees C for 7 or 30 d, respectively. Frozen storage or urine specimens with or without a preservative adversely affected the levels of urinary protein after 30 d or longer.
Studies
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Study Purpose
The purpose of this paper was to assess the stability of urinary proteins after storage at -20 (1-180 d), 4 (1-30 d), or 22 (1-7 d) degrees C in the presence or absence of a preservative that contained benzamidinium chloride, EDTA, tris (hydroxymethyl)-aminomethane, and azide.
Summary of Findings:
Concentrations of urinary proteins were comparable when specimens were stored at 22 or 4 degrees C for up to 7 or 30 d, respectively, with or without a preservative, with the exception of alpha1-antitrypsin which decreased by approximately 35% after storage in preservative-free specimens for the maximum duration (7 d at 22 degrees C, 30 d at 4 degrees C). Urine specimens stored at -20 degrees C for 180 d differed in IgG and alpha1-antitrypsin levels or lambda-immunoreactivity and alpha1-antitrypsin levels in preservative-treated and preservative-free specimens, respectively, compared to immediately processed controls. Alpha1-antitrypsin and IgG levels differed significantly from controls after 30 d or more of frozen storage.
Biospecimens
Preservative Types
- Frozen
- Other Preservative
- None (Fresh)
Diagnoses:
- Diabetes Type 1
- Other diagnoses
Platform:
Analyte Technology Platform Protein Immunoassay Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Preservation Type of fixation/preservation Frozen
None (fresh)
Refrigeration
Preservative cocktail
Storage Storage duration 1 d
3 d
7 d
14 d
30 d
90 d
180 d
Storage Storage temperature 22 degrees C
4 degrees C
-20 degrees C
Immunoassay Specific Targeted peptide/protein Albumin
Protein HC
IgG
Kappa-immunoreactivity
Lambda-immunoreactivity
Orosomucoid
Alpha1-antitrypsin