Stability of urinary female reproductive hormones stored under various conditions.

Author(s): Kesner JS, Knecht EA, Krieg EF Jr

Publication: Reprod Toxicol, 1995, Vol. 9, Page 239-44

PubMed ID: 7579908 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to assess the stability of female reproductive hormones (FSH, LH, E3G, Pd3G, creatinine) in urine specimens following storage at varying durations and temperatures and in the presence or absence of antimicrobial agents.

   Summary of Findings:

   Decreases in FSH, E3G, and creatinine urinary levels were observed overtime, with a more rapid decline and lower values observed at elevated temperatures (25 and 37 degrees C) after 1 week or more. For all analytes investigated, storage at or below 4 degrees C minimized loss of immunoreactivity compared to higher temperatures. The addition of antimicrobial agents to urine specimens attenuated loss of immunoreactivity in an analyte- and agent-specific manner when specimens were stored at -80 degrees C, with significant improvements in stability noted for FSH, E3G, and Pd3G with glycerol or LH with BSA. All other antimicrobial additives investigated were ineffective. Storage at -80 degrees C in the presence of glycerol or BSA preserved stability of urinary hormones for up to 24 weeks, with the exception of E3G, which exhibited a significant 10% decrease compared to baseline.

   Biospecimens

   • Bodily Fluid - Urine

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Steroid	Immunoassay
   Peptide	Immunoassay
   Protein	Spectrophotometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	-80 degrees C 4 degrees C 25 degrees C 37 degrees C
   Storage	Storage duration	0 weeks 1 week 2 weeks 7 weeks 24 weeks
   Biospecimen Preservation	Type of fixation/preservation	BSA Glycerol

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