Evaluation of blood collection tubes using selected reaction monitoring MS: implications for proteomic biomarker studies.

Author(s): Randall SA, McKay MJ, Baker MS, Molloy MP

Publication: Proteomics, 2010, Vol. 10, Page 2050-6

PubMed ID: 20209509 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of collection tube type and thaw duration on proteomic analysis using selected reaction monitoring MS. Plasma was stored at -140 degrees C and thawed at room temperature.

   Summary of Findings:

   There were slightly higher levels of 12 of the 18 peptides analyzed in specimens collected in tubes containing a protease inhibitor cocktail (P100) than specimens collected in PPT or EDTA tubes. Only Transthyretin show differential expression between PPT and EDTA tubes, while Kininogen I and Vitamin D-binding protein levels were different between P100 tubes and PPT, and Kininogen I, Transthyretin, Apolipoprotein A-I, and Vitamin D-binding protein levels were different between P100 and EDTA tubes. When analysis of plasma was delayed after thawing, significant declines in protein recovery were observed after 1 hour, regardless of tube type.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	LC-MS or LC-MS/MS
   Peptide	LC-MS or LC-MS/MS

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Type of collection container/solution	Vacutainer EDTA tube Vacutainer PPT P100
   Analyte Extraction and Purification	Protease inhibitor	No protease inhibitor added Cocktail
   Storage	Thaw duration	0 h 1 h 2 h 4 h 8 h

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