Evaluation of blood collection tubes using selected reaction monitoring MS: implications for proteomic biomarker studies.
Author(s): Randall SA, McKay MJ, Baker MS, Molloy MP
Publication: Proteomics, 2010, Vol. 10, Page 2050-6
PubMed ID: 20209509 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of collection tube type and thaw duration on proteomic analysis using selected reaction monitoring MS. Plasma was stored at -140 degrees C and thawed at room temperature.
Summary of Findings:
There were slightly higher levels of 12 of the 18 peptides analyzed in specimens collected in tubes containing a protease inhibitor cocktail (P100) than specimens collected in PPT or EDTA tubes. Only Transthyretin show differential expression between PPT and EDTA tubes, while Kininogen I and Vitamin D-binding protein levels were different between P100 tubes and PPT, and Kininogen I, Transthyretin, Apolipoprotein A-I, and Vitamin D-binding protein levels were different between P100 and EDTA tubes. When analysis of plasma was delayed after thawing, significant declines in protein recovery were observed after 1 hour, regardless of tube type.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Protein LC-MS or LC-MS/MS Peptide LC-MS or LC-MS/MS Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Acquisition Type of collection container/solution Vacutainer EDTA tube
Vacutainer PPT
P100
Analyte Extraction and Purification Protease inhibitor No protease inhibitor added
Cocktail
Storage Thaw duration 0 h
1 h
2 h
4 h
8 h