Proteomic analysis and comparison of the biopsy and autopsy specimen of human brain temporal lobe.

Author(s): He S, Wang Q, He J, Pu H, Yang W, Ji J

Publication: Proteomics, 2006, Vol. 6, Page 4987

PubMed ID: 16912969 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this paper was to optimize protein sample preparation for increased gel separation using 2 dimensional electrophoresis (2-DE).

   Summary of Findings:

   Sample treatment with DNase and RNase, ultracentrifugation, followed by acetone precipitation (Method 4) yielded superior 2-DE results, with both an increased number of protein spots and reduced background compared to other extraction protocols. The authors concluded that while treatment with DNase and Rnase, and acetone precipitation were required, ultracentrifugation and the Amersham clean-up kit marginally improved gel quality.

   Biospecimens

   • Tissue - Brain

   Preservative Types

   • Frozen

   Diagnoses:

   • Epilepsy
   • Autopsy
   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   Protein	1D/2D gels

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Analyte Extraction and Purification	Analyte isolation method	Pre-cooled acetone precipitation Amersham clean-up kit DNAse, RNAse, Amersham clean-up kit DNAse, RNAse, ultracentrifugation, acetone

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