Effects of freeze-thawing and storage on ultracentrifugal properties of human serum lipoproteins.
Author(s): DEL GATTO L, NICHOLS AV, LINDGREN FT
Publication: Proc Soc Exp Biol Med, 1959, Vol. 101, Page 59-61
PubMed ID: 13658190 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
-
Study Purpose
The purpose of this study was to determine the effects of freeze-thaw cycling, freezing and thawing rates, and storage on lipoprotein concentrations and Sf in serum from patients with high lipoprotein levels.
Summary of Findings:
Only minimal changes in lipoprotein concentrations and Sf were observed after 1 freeze-thaw cycle compared to specimens measured fresh, regardless of freezing and thawing rates. The concentration and Sf of the lipoprotein classes were not altered by 3 freeze-thaw cycles compared to 1 freeze-thaw cycle, regardless of freeze or thaw method. When specimens were stored at -30 to -26 degrees C, the abundance of lipoproteins with Sf above 20 decreased with increasing storage duration, but lipoproteins with Sf below 20 and HDL were relatively stable (<30% change). When stored between -5 and 0 degrees C or 0 and 4 degrees C, all lipoproteins were stable for at least 28 and 14 days, respectively, after which point the HDL peak broadened and the abundance of lipoproteins with Sf >20 decreased.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Other diagnoses
Platform:
Analyte Technology Platform Lipoprotein Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage temperature -30 to -26 degrees C
-5 to 0 degrees C
0 to 4 degrees C
Storage Storage duration 0 days
7 days
28 days
78 days
128 days
178 days
180 days
360 days
Storage Thaw temperature/condition 37 degree water bath
Room temperature
Storage Freeze/thaw cycling 0 cycles
1 cycle
2 cycles
3 cycles
Preaquisition Diagnosis/ patient condition Elevated lipoproteins
Biospecimen Preservation Cooling or freezing method/ rate Acetone dry ice bath
Deep freezer