Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Improvement in fetal DNA extraction from maternal plasma. Evaluation of the NucliSens Magnetic Extraction system and the QIAamp DSP Virus Kit in comparison with the QIAamp DNA Blood Mini Kit.

Author(s): Clausen FB, Krog GR, Rieneck K, Dziegiel MH

Publication: Prenat Diagn, 2007, Vol. 27, Page 6-10

PubMed ID: 17154236 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to compare the yield of cffDNA and total cell-free DNA from plasma using three different extraction methods. DNA was extracted from frozen (-30ºC) plasma of 75 Rhesus blood group D (RhD)-negative pregnant women who gave birth to RhD-positive infants. cffDNA and total cell-free DNA were quantified through real-time PCR amplification of RhD and glyceraldehyde 3-phosphate dehydrogenase (GAPDH), respectively.

   Summary of Findings:

   Extraction of cffDNA with the QIAamp DSP Virus Kit or the NucliSens Magnetic Extraction system resulted in 2.4- and 1.7-fold higher cffDNA yields per mL of plasma  in comparison to the QIAamp blood kit (p=0.00007 and p=0.00001, respectively). Further, yields of non-fetal cell free DNA per mL of plasma were 2.1- and 2.3-fold higher using the QIAamp DSP Virus Kit or the NucliSens Magnetic Extraction system, respectively, than the QIAamp blood kit (p=0.002 and p=0.039, respectively).

   Biospecimens

   • Bodily Fluid - Plasma

   Preservative Types

   • Frozen

   Diagnoses:

   • Pregnant

   Platform:

   Analyte	Technology Platform
   DNA	Real-time qPCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Real-time qPCR Specific	Targeted nucleic acid	GAPDH RhD
   Analyte Extraction and Purification	Analyte isolation method	QIAamp DSP Virus Kit NucliSens Magnetic Extraction system QIAamp DNA Blood Mini Kit

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