Efficient DNA extraction from 25-year-old paraffin-embedded tissues: study of 365 samples.

Author(s): Gillio-Tos A, De Marco L, Fiano V, Garcia-Bragado F, Dikshit R, Boffetta P, Merletti F

Publication: Pathology, 2007, Vol. 39, Page 345-8

PubMed ID: 17558863 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine if DNA extracted from formalin- or Bouin's-fixed archival paraffin blocks (stored for 22-26 years) can be successfully used for PCR analysis of differentially sized beta-globin amplicons. A total of 365 differentially sized specimens were investigated.

   Summary of Findings:

   Successful PCR analysis was influenced by the size of the generated amplicon and the size of the biospecimen used for DNA extraction. PCR success rates of 69%, 17%, and 5% were observed for amplicon sizes of 152 bp, 268 bp, and 676 bp, respectively. PCR success rate was significantly lower when large biospecimen sections were used for DNA extraction compared to medium or small, although terms were not defined by the authors. Fixative dependent effects (formalin versus Bouin's) were not significant, although analysis was limited to the 152 bp amplicon results and Bouin's fixed specimens were decolorized with Li2CO3 prior to DNA extraction.

   Biospecimens

   • Tissue - Larynx

   Preservative Types

   • Formalin
   • Other Preservative

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   DNA	PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   PCR Specific	Length of gene fragment	152 bp 268 bp 676 bp
   Biospecimen Preservation	Type of fixation/preservation	Bouin's fixative Formalin (buffered)
   Storage	Storage duration	22-26 yr
   Biospecimen Aliquots and Components	Aliquot size/volume	Small tissue section Medium tissue section Large tissue section

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