Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Tissue microarrays from HOPE-fixed specimens allow for enhanced high through put molecular analyses in paraffin-embedded material.

Author(s): Goldmann Torsten, Dromann Daniel, Marzouki Marouane, Schimmel Udo, Debel Karsten, Branscheid Detlev, Zeiser Tobias, Rupp Jan, Gerdes Johannes, Zabel Peter, Vollmer Ekkehard

Publication: Pathol Res Pract, 2005, Vol. 201, Page 599

PubMed ID: 16259114 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine if HOPE-fixed, paraffin-embedded tissues can be successfully used for tissue microarray detection of breast cancer markers (Ki-67, Her2) and TLR2 in lung.

   Summary of Findings:

   Tissue microarray detection of Ki-67, Her2, and TLR2 using HOPE-fixed paraffin embedded tissues yielded comparable results to those obtained with single tissue sections, and with formalin fixation, with a high degree of staining quality and well preserved morphological detail.

   Biospecimens

   • Tissue - Breast
   • Tissue - Lung

   Preservative Types

   • Other Preservative
   • Formalin

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Protein	Tissue microarray
   Protein	Immunohistochemistry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	HOPE-Fixation Formalin (buffered)
   Biospecimen Aliquots and Components	Type of slide	Whole tissue section Tissue microarray section

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2. Study Purpose

   The purpose of this paper was to determine if HOPE-fixed, paraffin-embedded tissues can be successfully used for tissue microarray in situ hybridization for TLR2 in lung.

   Summary of Findings:

   In situ hybridization signals for TLR2 in HOPE-fixed, paraffin-embedded tissue microarrays were distinct, intense, and appropriately localized.

   Biospecimens

   • Tissue - Lung

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Neoplastic

   Platform:

   Analyte	Technology Platform
   RNA	Tissue microarray
   RNA	In situ hybridization

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	HOPE-Fixation

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